期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

单核细胞趋化蛋白-1在EAE大鼠血清中的动态变化及意义 被引量：1

Dynamic changes of monocyte chemoattractant protein-1 expression in sera of experimental autoimmune encephalomyelitis rats and its significance
下载PDF
导出
摘要 目的探讨单核细胞趋化蛋白-1(monocyte chemoattractant protein-1,MCP-1)在实验性自身免疫性脑脊髓炎(ex-perimental autoimmune encephalomyelitis,EAE)大鼠血清中的动态变化及意义。方法以豚鼠脊髓匀浆(guinea pig spinal cord homogenate,GPSCH)加完全弗氏佐剂(complete freund’s adjuvant,CFA)作为抗原,给予Wistar大鼠四足皮内注射,建立EAE模型。采用双抗体夹心法酶联免疫吸附实验(ELISA)检测EAE大鼠免疫后7、14、21d不同时间段血清中MCP-1水平。结果EAE组免疫后第7天血清MCP-1水平即出现明显升高,早于临床症状的发生;免疫后第14天随着EAE大鼠进入症状高峰期,血清MCP-1水平亦达高峰;免疫后第21天MCP-1水平恢复到正常水平。与NS组和佐剂(CFA)组比较,EAE组大鼠免疫后第7、14天MCP-1水平显著性增高(P<0.01),并且EAE组大鼠免疫后第14天MCP-1水平与临床评分呈显著正相关(r=0.8993,P=0.0001)。结论MCP-1在EAE免疫学发病机制中具有重要作用,为深入研究多发性硬化(multiple sclerosis,MS)的发病机理及探索新的有效治疗途径提供了理论依据。 Objective To study the dynamic changes of monocyte chemoattractant protein-1 （MCP-1 ） in sera of experimental autoimmune encephalomyelitis （EAE） rats and its significance. Methods All experimental Wistar rats were inoculated intradermicly in the feet with emulsion containing guinea pig spinal cord homogenate （GPSCH） and complete Freund＇s adjuvant （CFA）. The level of serum MCP-1 in EAE rats was detected by sandwich-enzyme-linked immunosorbent assay （ELISA） on days 7, 14, and 21 post inlmunization. Results The level of serum MCP-1 in EAE group was increased on day 7 post immunization, which was prior to the onset of clinical symptoms. The level of serum MCP-1 reached its climax concurrently with symptom peak of EAE rats on day 14, and then grew to normal level on day 21. The level of serum MCP-1 of EAE group on day 7 and 14 was significantly higher than that of NS group and CFA group （ P 〈 0.01 ）. There was a significantly positive correlation between clinical scores and MCP-1 expression on day 14（ r = 0. 8993, P = 0. 0001 ）. Coneluslon MCP-1 plays an important role in the immunopathogenesis of EAE. This experiment offers theoretical evidence for further study on pathogenesis of multiple sclerosis （MS） and gives the clues for searching new effective therapeutic approaches of MS.
作者 徐玉 郭力 刘瑞春 赵平平 王秀丽 王颖 袁玉洁
机构地区 河北医科大学第二医院神经内科
出处 《免疫学杂志》 CAS CSCD 北大核心 2008年第5期519-521,526,共4页 Immunological Journal
基金 河北省科技攻关计划基金资助项目(03276403D-18)
关键词 实验性自身免疫性脑脊髓炎 单核细胞趋化蛋白-1 多发性硬化 Experimental autoimmune encephalomyelitis Monocyte chemoattractant protein-1 Multiple sclerosis
分类号 R744.51 [医药卫生—神经病学与精神病学]
  • 相关文献

参考文献13

  • 1Berman JW, Guida MP, Warren J, et al. Localization of monocyte chemoattractant peptide-1 expression in the central nervous system in experimental autoimmune encephalomyelitis and trauma in the rat [ J ]. J Immunol, 1996, 156 ( 8 ) : 3 017 - 3 023.
  • 2Kono DH, Urban JL, Horvath SJ, et al. Two minor determinants of myelin basic protein induce experimental allergic encephalomyelitis in SJL/J mice [J]. J Exp Med, 1988, 168(1) : 213 - 227.
  • 3Vin MC, Lee SM, Kalume F, et al. Autoimmunity due to molecular mimicry as a cause of neurological disease[J]. Nal Med, 2002, 8 (5): 509-513.
  • 4McManus C, Berman JW, Brett FM, et al. MCP-1, MCP-2 and MCP-3 expression in multiple sclerosis lesions: an immunohistochemical and in situ hybridization study[J]. J Neuroimmunol, 1998, 86 ( 1 ) : 20 - 29.
  • 5Van Der Voorn P, Tekstra J, Beelen RH, et al. Expression of MCP-1 by reactive astrocytes in demyelinating multiple sclerosis lesions[J]. Am J Pathol, 1999, 154 (1) : 45 - 51.
  • 6Franciotta D, Martino G, Zardini E, et al. Serum and CSF levels of MCP-1 and IP-10 in multiple sclerosis patients with acute and stable disease and undergoing immunomodulatory therapies[J]. J Neuroimmunol, 2001, 115 (1/2): 192- 198.
  • 7Iarlori C, Reale M, De Luca G, et al. Interferon beta-1b modulates MCP-1 expression and production in relapsing-remitting multiple sclerosis [ J ]. J Neuroimmunol, 2002, 123(1/2): 170- 179.
  • 8Juedes AE, Hjelmstrom P, Bergman CM, et al. Kinetics and cellular origin of cytokines in the central nervous system: insight into mechanisms of myelin oligodendrocyte glycoprotein- induced experimental autoimmune encephalo myelitis [ J]. J Immunol, 2000, 164 ( 1 ) : 419 - 426.
  • 9Youssef S, Wildbaum G, Maor G, et al. long-lasting protective immunity to experimental autoimmune encephalomyelitis following vaccination with naked DNA encoding C-C chemokines[J]. J Immunol, 1998, 161 (8) : 3 870- 3 879.
  • 10Huang DR, Wang J, Kivisakk P, et al. Absence of monocyte chemoattractant protein 1 in mice leads to decreased local macrophage recruitment and antigen-specific T helper cell type 1 immune response in experimental autoimmune encephalo-myelitis[J]. J Exp Med, 2001, 193 (6) : 713 - 726.

二级参考文献9

  • 1戚晓昆,蒲传强,朱克,关明臣,张凤英,刘洁晓,朱光明,张曙光,昝世明.实验性变态反应性脑脊髓炎模型病理及组化研究[J].中华神经科杂志,1996,29(2):97-99. 被引量：13
  • 2Kieserei B, Kiefer R, Ckements J, et al. Matrix metalloproteinase-9 and-7 are regulated in experimental autoimmune encephalomyelitis[J]. Brain, 1998, 121(1): 159-166.
  • 3Fu L, Matthews P, De Stefano N, et al. Imagine axonal damage of normal-appearing white matter in multiple sclerosis[J]. Brain, 1998, 121(1):103- 113.
  • 4Bitsch A, Schuchardt J, Bunkowski S, et al. Acute axonal injury in multiple sclerosis [ J ]. Brain, 2000, 123 (6) :1 174-1 183.
  • 5Pitt D, Wemer P, Raine C. Glutamate excitotoxicity as a mechanism in multiple sclerosis[J]. Nat Med, 2000, 6( 1 ):67 - 70.
  • 6Steinman L. Multiple sclerosis: a two stage disease[J]. Nat Med, 2001, 2(9) :762 - 765.
  • 7Miller A, Galboiz Y. Multiple sclerosis: from basic immunopathology to immune intervention[J]. Clin Neural Neurosurg,2002, 104(1) : 172 - 176.
  • 8张浩,朱平.自身免疫病的Th1/Th2细胞因子平衡治疗[J].免疫学杂志,2000,16(2):119-122. 被引量：38
  • 9夏斌,张玲珍,邵福源,赵忠新.多发性硬化患者外周血淋巴细胞CD56的表达[J].免疫学杂志,2001,17(5):376-378. 被引量：1

共引文献6

同被引文献9

  • 1Youssef S,Stuve O,Patarroyo JC,et al. The HMG- CoA reduetase inhibitor , atorvastatin , promotes a Th2 bias and reverses paralysis in central nervous system autoimmune disease [ J ]. Nature, 2002, 420 (6911) ：78 - 84.
  • 2Kobashigawa JA, Katznelson S, Laks H, et al. Effect of pravastatin on outcomes after cardiac transplanta- tion[J]. N Engl J Med,1995,333(10) ：621 - 627.
  • 3Diestel A, Aktas O, Hackel D, et al. Activation of mi- croglial poly(ADP- ribose) - polymerase- 1 by cho- lesterol breakdown products during neuroinflamma- tion., a link between demyelination and neuronal damage[J]. J Exp Med, 2003,198(11) : 1729 - 1740.
  • 4Zamvil SS. Steinman L Cholesterol- lowering statins possess antiinlammatory activity that misht be useful for treatment of MS[J] Neurology, 2002,59： 970 - 971.
  • 5Nath N,Giri S,Prasad R,et al. Potential target s of 3 - hydroxy - 3 - methylglutaryl coenzyme A reduc- tase inhibitor for multiple sclerosis therapy [J]. J Im- mu- nol,2004,172(2) ：1273 - 1286.
  • 6Juedes AE, HjelmstromP, Bergman CM, et al. Kinet- ics and cellular origin of cytokines in the central nervous system: insight into mechanisms of myelin oligodendrocyte glycoprotein induced experimental autoimmune encephalo myelitis[J]. J Immunol,2000, 164 (1) ： 419- 426.
  • 7Huang DR, Wang J, Kivisakk P, et al. Absence of monocytochemoattractant protein i in mice leads to decreased local macrophage recruitment and antigen specific T helper cell type 1 immune response in ex- perimental autoimmune encephalomyelitis[J]. J Exp Med,2001,193:713 - 726.
  • 8Godiska R, Chantry D, Dietsch GN, et al. Chemokine expression in murine experimental allergic encephalo- myelitis[J]. J Neuroimmunol, 1995,58 (2) ： 167 - 176.
  • 9徐玉,刘瑞春,王颖,赵平平,王秀丽,刘静,郭力.Wistar大鼠实验性自身免疫性脑脊髓炎模型的建立[J].脑与神经疾病杂志,2007,15(6):415-417. 被引量：15

引证文献1

免疫学杂志
2008年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部