不同出生体重新生大鼠胰腺ghrelin和钙通道表达的研究

Expression of ghrelin and calcium channels in pancreas of neonatal rats with different birth weight

目的:探讨宫内营养环境对大鼠胰腺ghrelin和胰岛细胞团上L型钙通道(Cav1.2、Cav1.3)相对丰度的影响。方法:建立不同出生体重新生大鼠模型,分别取新生第1天大鼠胰腺或分离的胰岛细胞团组织,用实时荧光定量RT-PCR观察不同出生体重组胰腺ghrelin mRNA表达的变化和胰岛细胞团上Cav1.2、Cav1.3 mRNA的差异;同时,对胰岛细胞团上ghrelin、Cav1.2、Cav1.3蛋白进行免疫组织化学染色。结果:实时荧光定量RT-PCR显示:新生大鼠小于胎龄儿(SGA)组胰腺ghrelin mRNA水平高于适于胎龄儿(AGA)组的表达(P<0.05)。胰岛细胞团上Cav1.2 mRNA SGA组和大于胎龄儿(LGA)组表达量均小于AGA组,Cav1.3 mRNA SGA组小于AGA组(P<0.05)。免疫组化积分光密度数据显示与实时荧光定量RT-PCR结果呈相同趋势。结论:早期宫内营养状况过剩或缺乏可能导致胰腺分化和成熟障碍,并引起胰岛细胞团上ghrelin和钙通道表达差异。

Objective： To investigate the effect of the intrauterine environment on pancreas ghrelin and L-type calcium channels of islet like cell clusters （ICCs） in neonatal rats. Methods： Different birth weight neonatal rat models were established. The samples of entire pancreas or ICCs were collected at the first day after birth. Ghrelin,Cavl. 2, Cavl. 3 mRNA were determined using real time RT PCR and immunohistochemistry respectively. s and Resu real-time RT-PCR revealed that ghrelin mRNA in SGA group were significantly higher t proteins Its ： The han that of the AGA group （P（0.05）. Cavl. 2 mRNA of SGA group and LGA group was significantly lower than that of the AGA group; Cavl. 3 mRNA of SGA group was significantly lower than that of the AGA group（P（0.0S）.The results of integral OD value of immunohistochemistry were consistent with those of real-time RT-PCR. Conclusion. Intrauterine nutritional status may affect pancreatic endocrine cells differentiation and maturity, resulting in the difference of expression ghrelin and calcium channels in ICCs.