盐酸吗啡对肠道运动的离体与在体作用及机制研究

Effect of morphine chloride on contractility of small intestinal muscle in vitro or in vivo and its mechanisms

目的:研究盐酸吗啡对肠道平滑肌运动的影响及其作用机制。方法:制备家兔离体小肠,记录小肠平滑肌发展张力、静止张力、收缩频率,观察盐酸吗啡对肠肌运动的影响。并进一步测定不同浓度的盐酸吗啡灌胃前后小鼠小肠推进运动的变化。结果:5 m g/L、10 m g/L、30 m g/L的盐酸吗啡作用于离体肠肌,对家兔小肠发展张力均有明显的抑制作用(P<0.05)。纳洛酮可削弱盐酸吗啡对肠肌发展张力的抑制作用,吗啡对肠肌发展张力的抑制作用从78.7%±13.4%至87.8%±11.2%(P<0.05)。阿托品可阻断盐酸吗啡对肠肌发展张力的抑制作用,吗啡对肠肌发展张力的抑制百分比从77.2%±12.1%至103.7%±12.8%(P<0.05)。而酚妥拉明则增强盐酸吗啡对肠肌发展张力的抑制作用,吗啡对肠肌发展张力的抑制百分比从79.2%±11.8%至69.8%±15.3%(P<0.05)。用不同浓度(75、150、300 m g/L)的盐酸吗啡灌胃后小鼠小肠推进均减慢,推进率分别为54.9%±15.5%、47.7%±14.3%、37.1%±5.8%,与生理盐水灌胃组比较有显著性意义(P<0.05)。结论:盐酸吗啡在离体与在体水平对肠肌运动均有抑制作用,其机制可能与阿片受体、胆碱能受体、肾上腺素能受体有关。

Objective： To investigate the effect of morphine chloride on small intestinal muscle in vitro or in vivo and its mechanisms. Methods： Contractile amplitude,tension and frequency of the isolated small intestine of rabbits were measured before and after treatment of morphine chloride. The propulsive distance of magenta in intestinal tract was measured when different concentration of morphine chloride was given orally in mice. Results.. After treatment of different concentration of morphine chloride （5 mg/L., 10 mg/L, 30 mg/L）, the contractile activities of isolated small intestines of rabbits decreased significantly. The inhibitory effect of morphine chloride was blocked by naloxone,atropine,but potentiated by regitine. The propulsive distance of magenta in intestinal tract of intact mouse decreased after treatment with morphine chloride of various concentration （75,150,300mg/L）. Conclusions： Morphine chloride has an inhibitory effect on the contractility of rabbit small intestine in vitro or in vivo. Opioid receptor,choline and adrenal receptor might be involved in this effect.