不同超促排卵方案对子宫内膜性激素受体和白血病抑制因子表达的影响

Expression of estrogen receptor,progesterone receptor and leukemia inhibitory factor on endometrium during different ovarian stimulation protocols in mice

目的:探讨促性腺激素释放激素激动剂(GnRHa)长方案和单纯促性腺激素(PMSG)超促排卵对分泌期子宫内膜雌、孕激素受体和白血病抑制因子(LIF)表达的影响。方法:以小鼠为超促排卵动物模型,采用GnRHa+PMSG长方案促排卵,以单用PMSG促排卵和自然周期为对照,在排卵后取子宫内膜,免疫组化定量测定腺上皮细胞雌、孕激素受体表达水平;逆转录-聚合酶链反应(RT-PCR)半定量检测内膜LIF mRNA表达。结果:免疫组化显示:GnRHa+PMSG组在注hCG后内膜腺上皮细胞ER、PR免疫组化阳性细胞百分率为(88.46±2.51)%、(84.71±2.21)%,染色强度为(0.415±0.048)AU、(0.454±0.046)AU,均低于自然周期组同期值,分别为(92.36±2.33)%、(87.82±1.22)%和(0.602±0.051)AU、(0.542±0.055)AU(P<0.01);但高于单纯PMSG组,分别为(82.62±1.63)%、(74.19±1.39)%和(0.366±0.044)AU、(0.364±0.028)AU(P均<0.05)。RT-PCR显示:GnRHa+PMSG组注入hCG后内膜LIF mRNA表达相对量(1.703±0.549)高于单纯PMSG组(1.535±0.641,P<0.01),但两者均低于自然周期组(1.959±0.778,P<0.01)。结论:GnRHa长方案辅助超促排卵可影响子宫内膜性激素受体和LIF的表达水平,可能对内膜容受性有一定影响,但优于单纯促性腺激素方案。

[-Abstract] Objective： To evaluate the influence of superovulation by GnRHa protocol and pregnant mare＇s serum gonadotropin （PMSG） alone on the expression of estrogen receptor （ER） ,progesterone receptor （PR） and leukemia inhibitory factor （LIF） mRNA on endometrium. Methods： Forty-five femal ICR mice were randomly allocated into 3 groups： （1） GnRHa ＋PMSG group ：alarelin was give first for desensitizing the pituitary,then superovulation with PMSG; （2） PMSG group ：mice were injected with PMSG only; （3） Natural cycle group ：mice were given with same volume of saline. Endometrium samples were taken at 48 hours after given hCG or ovulation （control group）. ER and PR in glandular cells were detected with SP immunohistochemistry semiquantitatively. Expression of LIF mRNA on endometrium was detected with reverse transcriptase-polymerase chain reaction （RT-PCR） analysis. Results. The positive rate（%） and expression intense （AU） of ER and PR on glandular epithelium cells were significantly lower in GnRHa＋PMSG group and PMSG group than those in natural cycle group （all P〈0.01）. The expression of LIF mRNA was significantly lower in GnRHa ＋PMSG group and PMSG group than that in natural cycle group （all P〈0.01）; but the expressions of ER,PR and LIF in GnRHa＋ PMSG group were higher than those in PMSG group. Conclusion： The protocol with GnRHa down regulates the expressions of ER,PR and endometrium,suggesting it may have an adverse it may still be better than PMSG alone. the LIF mRNA on the mice of secretive phase effect on the endometrial receptivity in mice,but