经微弧氧化表面改性的钛铌锆锡合金的细胞相容性评价

Biocompatibility Evaluation of Osteoblast on Ti-24Nb-4Zr-7.9Sn after Treated with Micro-arc Oxidation.

目的:探讨采用微弧氧化技术(micro-arc oxidation,MAO)处理钛铌锆锡合金(Ti-24Nb-4Zr-7.9Sn,TZNS)对成骨细胞在其表面生长的影响。方法:采用MAO方法处理TNZS,利用扫描电镜、表面轮廓测量仪和接触角测量仪,分别研究其形貌特点、表面粗糙度和表面能大小;将原代培养的成骨细胞接种于材料表面,培养1、4、7、10d,扫描电镜观察成骨细胞在不同表面上粘附形态的变化,并用MTT法对各组细胞培养数量进行定量检测。结果:微弧氧化处理在材料表面形成了一层多孔、粗糙、凸凹不平的氧化膜,试样的表面粗糙度和表面能较未处理组均增加(P<0.05);成骨细胞培养结果表明,在两组材料表面细胞均生长良好,扫描电镜观察细胞为多边形,胞浆丰富,有多个伪足突起呈分化表型,统计学分析结果显示,在各个时间点上,实验组(MAO-TNZS)细胞数量较对照组高,差异有显著性(P<0.05)。结论:经微弧氧化处理的钛铌锆锡合金,具有良好的成骨细胞相容性,在体外细胞培养的环境下,有利于细胞的附着和增殖。

Objective： To investigate biocompatibility of cultured osteoblast on Ti -24Nb-4Zr-7.9Sn（TNZS） alloy treated with micro - arc oxidation （MAO） in vitro. Methods： The TNZS alloy was treated with MAO, scanning electron microscopy （SEM） was used to characterize surface microtopograph, surface roughness was measured by a profilometer and the goniometer was used to evaluate the contact angle and surface energy. Primary osteoblast culture was established from parietal and frontal bones of SD rats, which was then. inoculated onto specimens of MAO - TNZS and untreated TNZS. The morphology of the cells was observed by scanning electron microscopy （SEM） and the amount of cells was surveyed by MTT method at 1d, 4d, 7d and 10d respectively. Results： The Micro - arc oxidation treatment produced a layer of porous oxide coating on the surface of TNZS discs, the surface roughness and surface energy of MAO - TNZS were significantly higher than that of smooth TNZS. As the cultured time increased, the cells proliferated well and secreted plenty of matrix, SEM observation showed that the adhesion between cells and materials were pretty well, the statistic results indicated that the multiplication of MAO- TNZS was significantly higher than that of Ti specimen （P 〈 0.05 ）. Conclusion： The Ti -24Nb -4Zr- 7.9Sn after treated with micro-are oxidation possesses good biocompatibility to osteoblast.