牙龈卟啉单胞菌对纯钛表面成骨细胞表达OPG和RANKL mRNA的影响

Effects of Porphyromonas Gingivalis on the Expression of OPG and RANKL mRNA Level in Osteoblasts on Titanium.

目的:在转录水平上观察牙龈卟啉单胞菌对成骨细胞表达OPG及RANKL mRNA的影响。方法:用浓度分别为106、18CFU/mL的牙龈卟啉单胞菌刺激接种于纯钛表面的成骨细胞,24 h后提取各组成骨细胞总RNA并应用逆转录-聚合酶链式反应和mRNA比色定量的方法检测OPG及RANKL基因表达。结果:MG-63成骨细胞基础表达较强的OPG mRNA及少量的RANKL mRNA,牙龈卟啉单胞菌以浓度依赖的方式增强RANKL mRNA的表达,减弱OPG mRNA的表达。结论:牙龈卟啉单胞菌通过上调RANKL/OPG的比率可间接的调节破骨细胞的活性从而影响支抗种植体周的骨代谢。

Objective ： To evaluate the effects of Porphyromonas gingivalis on the mRNA expression of OPG and RANKL at transcription level in osteoblasts. Methods： Porphyromonas gingivalis at a density of 10^6, 10^8 CFU/ml were added to the osteoblasts seeded on the surface of pure titanium. The total RNA was extracted after 24h co - culture and mRNA expression of OPG and RANKL was examined through reverse transcription - polymerase chain reaction and mRNA colorimetric quantification, Results： MG- 63 expressed OPG and RANKL mRNA constructively, while Porphyromonas gingivalis decreased the mRNA expression of OPG and increased that of RANKL dose - dependently. Conclusion ： Porphyromonas gingivalis had the effects on the activities of osteoblasts by regulating the ratio of OPG/RANKL.