6-苄氨基嘌呤和乙烯利对小麦叶片碳素输出和分配的调节

REGULATION OF 6 BENZYLAMINOPURINE AND ETHYREL ON CARBO ASSIMILATES EXPORT FROM LEAF AND PARTITION IN WHEAT PLANT

在盆栽条件下，采用活体测量和定期取样测定的方法，研究了６苄氨基嘌呤（ＢＡ）和乙烯利（ＥＴＨ）处理对小麦幼苗第１茎生叶１４Ｃ同化物输出动态和向各器官中分配的影响。结果表明：饲喂后４８小时内，叶片１４Ｃ输出占引入量的９０％以上，叶片１４Ｃ同化物输出动态符合负指数方程（ｙ＝０．７１６ｅ－０．０５０６ｔ，ｒｔ，ｌｎｙ＝－０９６２３）。ＢＡ和ＥＴＨ处理未改变叶片中１４Ｃ同化物输出的大体趋势，输出动态曲线分别为ｙ＝０７３１９ｅ－００４７１ｔ（ｒｔ，ｌｎｙ＝－０９６７６）、ｙ＝０６９１２ｅ－００５６７ｔ（ｒｔ，ｌｎｙ＝－０９５５５），叶片１４Ｃ输出５０％需要的时间大小次序为：ＢＡ（８１ｈ）＞ＣＫ（７１ｈ）＞ＥＴＨ（５７ｈ）。ＣＫ、ＢＡ、ＥＴＨ处理的叶片１４Ｃ同化物输出速率（ｖ）随时间的变化方程分别为ｖ＝００３６３ｅ－００５０６ｔ、ｖ＝００３４５０ｅ－００４７１ｔ、ｖ＝００３９２ｅ－００５６７ｔ，最大输出速率（Ｖｍａｘ）的大小次序为：ＥＴＨ（３９２％／ｈ）＞ＣＫ（３６３％／ｈ）＞ＢＡ（３４５％／ｈ）。表明ＢＡ抑制而ＥＴＨ促进了１４Ｃ同化物从叶片中的输出。定期取样?

The regulation effects of 6 benzylaminopurine (BA, 0.05g/L) and ethyrel (ETH, 0.5g/L) on export of carbo assimilates from wheat leaf and its partition in the whole plant were studied using 14 CO 2 labeling method. Within 48 hours after labeling, more than 90% of the 14 C assimilates were exported from the labeled leaf. The kinetic equation of 14 C remained in labeled leaf treated by BA, ETH and control were y=0.716e -0.0506t (r t,lny =-0.9623 ** ), y=0 7319e -0.0471t (r t,lny =-0.9676 ** ), y=0.6912e -0.0567t (r t,lny =-0.9555 ** ), respectively. The duration for 14 C to reduce to 50% of the total 14 C were 8.1h (BA),7.1h (CK) and 5.7h (ETH). The exporting equatons of 14 C from labeled leaf with different treatments were V BA =0.0363e -0.0506t , V CK =0.03450e -0.0471t , V ETH =0.0392e -0.0567t . The maximum rate of export were increased by ETH and decreased by BA. It indicated that neither BA nor ETH changed the trends of 14 C assimilate export and BA decreased the rate of 14 C assimilates export from labeled leaf. Therefore 14 C assimilates remained in leaf was higher, while ETH acted quite different. In the total 14 C assimalates of wheat seedling, 50%～60% partitioned in newborn leaf, 20% was changed into structural compounds, 10% partitioned in roots, 10% was hold by stem and tiller node and little was exported to leaves and sheaths below the labeled leaf. BA retarded 14 C assimilates export from the labeled leaf. Both the 14 C assimilates partitioned in newborn leaf and leaves (including sheaths) below the labeled leaf were decreased. Export of 14 C assimilates to roots were retarded by both BA and ETH. \ \