人牙周韧带细胞与壳聚糖-胶原支架体外培养的实验研究

Effect of Chitosan/collagen Scaffold on Periodontal Ligament Cell Culture in Vitro.

目的:探讨壳聚糖-胶原支架在人牙周韧带细胞(PDLC)体外培养中的作用。方法:将体外培养PDLC接种于壳聚糖-胶原支架上,通过MTT法测定细胞增殖情况,通过半定量PCR检测细胞mRNA合成变化。结果:与传统的平板培养相比,PDLC在壳聚糖-胶原支架上的增殖及mRNA合成情况有显著性差异(P<0.05)。结论:该复合支架可以作为PDLC的载体;对PDLC的增殖及细胞外基质的合成均有较好地促进作用。

Objective： To study chitosan/collagen scaffold and observe the biological active of human periodontal ligament cell cultured on the scaffold in vitro. Methods： PDLC were seed on scaffold. Its proliferation was examined by MTT method and the effect of scaffold on mRNA synthesis was examined by semi - quantitive RT - PCR. Results： In contrast to control cultivation, on the proliferation and ECM mRNA synthesis of PDLC there had when cultured on the scaffolds. Conclusion： As a good carrier system, the complex scaffolds have significant effects on the proliferation of PDLC and ECM synthesis.