CK10、Bcl-2表达与牙源性角化囊肿复发关系的探讨

Relationship between the Recurrence of Odontogenic Keratocysts and their Expression of Cytokeratin 10 and Bcl-2 Protein.

目的:探讨CK10及Bcl-2的表达与牙源性角化囊肿(odontogenic keratocyst,OKC)复发的关系。方法:将OKC石蜡标本随机分为未复发组、复发初诊组、复发组,每组各10例,分别行CK10及Bcl-2免疫组化染色,并利用SPSS10.0统计软件进行列联表χ2检验。结果:3组病变CK10阳性表达率均为80%(8/10);未复发组Bcl-2阳性表达率为60%(6/10),其余两组为80%(8/10),且着色更深,除基底层外,阳性细胞尚可见于副基底层。结论:OKC复发前后及其与未复发的OKC之间上皮细胞的分化及成熟程度不存在明显差异,Bcl-2表达可能对OKC的预后具有一定作用。

Objective： To explore the relationship between the recurrence of odontogenic keratocysts and their expression of cytokeratin 10 （CK10） and Bcl-2 protein. Methods： The paraffin- embeded tissue sections of odontogenic keratocyst （OKC） were randomly divided into three groups： non - recurrent OKC （n = 10） , primary OKC （n = 10） , and recurrent OKC （n = 10 ）. The immunohistochemical expression of CK10 and Bcl -2 was studied using a modified biotin - streptavidin- peroxidase method on these sections, and the results of immunostaining were analyzed with SPSS 10.0. Results： The positive rates of CK10 in the three groups were 80 per cent （8/10）. The positive rate of Bcl -2 was 60 per cent （6/10） in non -recurrent OKC group, and 80 per cent （8/10） in another two groups. The positive staining of Bcl -2 in primary group and recurrent group was seen in the parabasal layer besides in the basal layer. Conclusion： There is no significant difference in the differentiation and mature extent of epithelium cells among non - recurrent OKC, primary OKC and recurrent OKC. Bcl -2 may play certain role for the prognosis of OKC.