水稻QTL图位克隆的特征分析

Analysis of features of 15 successful positional cloning of QTL in rice

图位克隆作为基因克隆的最有效手段,在水稻QTL克隆方面取得了很大的进展。文章通过分析近年来关于水稻QTL图位克隆的15个成功案例,总结了水稻QTL图位克隆的几个重要特征:(1)亲本杂交类型为种间或亚种间杂交,双亲的目标性状差异显著;(2)目标QTL为主效,一般能解释大部分的表型变异;(3)物理图距一般小于40kb;(4)初级定位结果准确,精细定位群体大于6000(隐性群体不小于1500)单株。文章还对QTL图位克隆的难点及解决方法进行了有益的探讨。

As the most efficient strategy in gene clone, positional cloning has been used widely in QTL cloning in rice. The objective of this paper is to make summary features of QTL positional cloning based on 15 successful positional cloning attempts. （1） most of the populations used in the analysis were derived from interspecific or intersubspecies; （2） the target QTL had been identified with very large phenotypic effects; （3） the candidate genomic region was usually narrowed down to 40 kb; （4） the primary mapping result was exact; and the fine mapping population was more than 6 000, while the number of recessive population was more than l 500. Otherwise, the nodus of QTL positional cloning and the corresponding solving methods were discussed.