摘要
用酸性聚丙烯酰胺凝胶电泳分离、表征４个小麦品种醇溶蛋白组成，并分析其Ｆ１，Ｆ２代２０个醇溶蛋白组分（带）的遗传模式。结果表明，醇溶蛋白呈共显性遗传，在２０个组分中，１６个组分由１个显性基因支配，２个组分则受控于２个独立基因，其余２个微小组分，由于在电泳谱上难以准确辨认，其遗传模式未能确定。有５对醇溶蛋白组分是共同遗传，表明每组组分由紧密连锁基因控制。另外，７对醇溶蛋白组分或组分群分别由等位位点的不同等位基因支配。
Gliadin proteins from four wheat cultivars were separated and characterized by using acid polyacrylamide gel electrophoresis (A PAGE, lactate acid, pH 3.1). Through analyzing F 1 and F 2 generation populations of two crosses (Temu 2065×Florida and Skopjanka×Lepoklasa), genetic models of 20 gliadin components (bands) were studied. The results showed that gliadin components are inherited according to the codominant type. Among 20 gliadin bands investigated, 16 are controlled by single dominant genes while 2 others are coded by two independent genes. For the remaining 2 bands, their genetic models were not determined because they are difficult to identify on the electrophoregrams. Five pairs of gliadin bands are always inherited together, suggesting that each may represent closely linked genes, and seven pairs of bands or groups appear to be controlled by different allelic loci.
出处
《西南农业大学学报（自然科学版）》
CSCD
1997年第5期409-414,共6页
Journal of Southwest Agricultural University
关键词
蛋白
遗传分析
小麦
醇溶蛋白
杂种二代
protein
electrophoresis
genetic analysis
triticum aestivum/gliadin
