粘蛋白MUC1 568A/G SNP与辽宁地区人群胃癌遗传易感性的关系

The relationship between the polymorphism of MUC1 and suscepti-bility to gastric cancer in Liaoning region

为了探讨粘蛋白(MUC1)基因568位点A/G单核苷酸多态性与胃癌遗传易感性的关系,采用序列特异性引物-聚合酶链反应(Sequence specific primers PCR,PCR-SSPs)检测来自辽宁地区人群138例胃癌患者及与其配比的131例对照个体MUC1568位点A/G多态性,以ELISA法检测血清H.pyloriIgG抗体。结果显示:(1)对照人群MUC1基因568位点AA、AG、GG3种基因型分布频率分别为73.3%、22.1%、4.6%;(2)胃癌组MUC1AA基因型携带频率显著高于正常对照组(P=0.03),携带MUC1AA基因型个体胃癌的发病风险增高到1.92倍;(3)以MUC1AG+GG基因型并血清幽门螺杆菌(H.pylori)IgG抗体阴性的个体为对照,AG+GG基因型并H.pyloriIgG抗体阳性个体、AA基因型并H.pyloriIgG抗体阴性个体、AA基因型并H.pyloriIgG抗体阳性个体胃癌患病风险增高,但3组各组间差异均无统计学意义(P>0.05)。说明MUC1基因568位点A/G多态与胃癌的遗传易感性相关;MUC1A/G基因多态性和H.pylori感染在胃癌发生发展过程未见交互作用。

Analyzed the relationship between 568 site A/G polymorphism in mucin I（MUC1） gene in a population from Liaoning Province and susceptibility to gastric cancer. Sequence specific primers-polymerase chain reaction（PCR-SSPs） were performed to analyze the genotype of the A/G polymorphism in its 568 site of exon 2 for 138 gastric cancer cases and 131 normal ones tested, and ELISA was performed to test IgG antibody of H. pylori. We found that the distribution fre- quency of AA, AG, GG three genotypes were 73.3%, 22.1%, 4.6%, respectively. The frequency of AA genotype was statis- tically higher in the gastric cancer （GC） group compared to the control group（P=0.03）, moreover, individuals with the MUC1 AA genotype increased 1.92 fold risk for gastric cancer. And compared with subjects carrying both AG＋GG geno- type and H. pylori-IgG negative, there was an obviously increased risk of developing gastric cancer for those who carried both AA genotype and H. pylori-IgG negative, both AG＋GG genotype and H. pylori-IgG positive, and both AG＋GG geno- type and H. pylori-IgG negative, but there were no significantly differences between the every two of the latter three. Our results indicated that there was a relationship between MUC1 A/G polymorphism and susceptibility to gastric cancer. And there were probably no statistically interaction between the 568 site A/G polymorphism in MUC1 and H. pylori infection in the occurrence and development process of gastric cancer.