基质金属蛋白酶-2在胆管癌组织中的表达及与预后的关系

Expression of MMP-2 in cholangiocarcinoma and its correlation with prognosis

目的:探讨基质金属蛋白酶-2(MMP-2)在胆管癌(CCA)组织中的表达及与预后的关系.方法:随机选取CCA患者106例,应用SP免疫组化方法检测MMP-2蛋白的表达.其中89例术后随访1～60mo,分析MMP-2蛋白与CCA患者预后的相关性.结果:MMP-2蛋白在CCA组织中的阳性表达率为76.42%(81/106),并与CCA组织的临床病理学特征及患者的预后均有密切的关系.在发生肿瘤浸润、淋巴道转移及低分化的肿瘤组织中,MMP-2蛋白高表达.此外,MMP-2蛋白阳性表达的CCA患者的生存率明显低于阴性表达的患者(P<0.01).结论:MMP-2蛋白的表达可能与CCA的浸润、转移有关;检测MMP-2蛋白在CCA组织中的阳性表达率,并结合其临床病理学分级,可提高对患者预后的判断准确性.

AIM： To investigate the clinicopathological features of the expression of matrix metalloproteinase-2 （MMP-2） in cholangiocarcinoma （CCA）, and to evaluate its clinical significance in the histologic classification and prognosis of patients with CCA. METHODS： 106 patients with CCA were examined for MMP-2 expression by SP immunohistochemistry. Then, the clinical outcome of the patients after a median follow-up of 1 - 60 months was determined in 89 out of the 106 patients. RESULTS： The positive rates of MMP-2 in CCA were 76.42% （81/106）. Clinicopathological parameters of CCA and clinical outcome of the patients were associated with MMP-2 expression. MMP-2 was expressed more frequently in CCA tissues with tumor invasion, poor differentiation and lymph node metastasis. Moreover, the survival rate of the patients with CCA expressing MMP-2 was significantly lower than that of the patients without MMP-2 expression （P 〈 0.01 ）. CONCLUSION： MMP-2 expression in cancerous tissues may play an important role in invasion and metastasis of CCA. The detection of it along with clinical staging may help to predict patients＇ prognosis.