上皮性卵巢癌患者外周血Th1/Th2细胞因子表达水平的变化

Levels of Thl and Th2 type cytokines in peripheral blood of patients with epithelial ovarian cancer

目的:探讨上皮性卵巢癌患者外周血Th1/Th2细胞因子表达水平.方法:用刺激物刺激细胞,增加细胞内细胞因子的表达,再加入荧光标记的特异性抗细胞因子单克隆抗体,流式细胞仪检测特异性细胞因子表达水平.结果:上皮性卵巢癌患者Thl型细胞因子IL-2,IFN-γ表达水平依次为(9.47±3.56)ng/L,(10.12±2.51)ng/L,而正常对照组依次为(12.20±2.48)ng/L,(13.38±2.89)ng/L,差异均有统计学意义(P<0.05);而Th2型细胞因子IL-4,IL-10表达水平依次为(4.56±0.78)ng/L,(5.26±2.27)ng/L,而正常对照组依次为(1.24±0.39)ng/L,(1.46±0.52)ng/L,两组差异有统计学意义(P<0.05).结论:卵巢癌患者Thl/Th2失调,Th1型反应弱,Th2型反应优,可使肿瘤细胞发生免疫逃逸.

AIM： To detect the levels of Thl and Th2 type cytokines in peripheral blood of patients with epithelial ovarian cancer, METHODS： The peripheral blood lymphocytes were stimulated to enhance the expression of cytokines, Then specific fluorescent-labeling monoclonal antibody was added, The expression of the cytokines was determined by flow cytometry, RESULTS ： The levels of IL-2 [ （ 9, 47 ± 3.56 ） ng/L] and IFN-γ[ （ 10.12 ± 2, 51 ） ng/L] produced by Thl cells were lower in patients with ovarian cancer than those in normal controls [ （ 12.20 ± 2.88）, （ 13. 38 ± 2. 89） ng/L, respectively] and the difference was significant （ P 〈 0, 05 ）. The levels of Th2 type cytokines, such as IL-4 and IL-10 were higher in patients with ovarian cancer [ （4.56 ±0.78）, （5.26 ±2.27） ng/L, respectively]than those in normal controls [ （ 1.24 ± 0.39 ）, （ 1.46 ± 0.52） ng/L. respectively ] with significant difference （ P 〈 0. 05 ）, CONCLUSION ： Th 1 / Th2 is unbalanced in ovarian cancer, and Thl type cytokines are inhibited, and Th2 type cytokines are relatively enhanced, so Th1/Th2 shifts to Th2, It may be the mechanism of tumor genesis and metastasis through immune escape from immunosurveillance.