复方酸枣仁油栀子油对小鼠的镇静、催眠和抗惊厥作用

Sedative,hypnotic and antiseizure effects of compound gardenia oil and jujube seed oil in mice

目的观察酸枣仁油、栀子油复合物的镇静、催眠、抗惊厥作用及二药的相互作用。方法利用光电计数法观察灌胃给予复方酸枣仁油栀子油对小鼠自发活动的影响,以及长期使用药物对小鼠耐受性的影响;利用延长戊巴比妥钠睡眠时间实验和戊巴比妥钠阈下催眠剂量实验,观察复方酸枣仁油栀子油的催眠作用;利用戊四氮诱发小鼠阵挛性、强直性惊厥观察复方酸枣仁油栀子油的抗惊厥作用;本实验用地西泮进行平行对照实验,并进行了组方设计和实验。结果酸枣仁油、栀子油及其复方可抑制小鼠自主活动;与地西泮相比,复方酸枣仁油栀子油起效较慢,随用药时间延长镇静作用逐渐增强,且无耐受现象;酸枣仁油、栀子油及其复方有协同催眠剂量戊巴比妥钠的催眠作用,可诱导阈下催眠剂量戊巴比妥钠小鼠产生睡眠;组方研究表明,复方酸枣仁油栀子油产生的镇静催眠作用较单独使用酸枣仁油、栀子油的作用强,出现明显的协同作用;未发现酸枣仁油、栀子油及其复方具有抗惊厥作用。结论复方酸枣仁油栀子油组方合理,具有镇静、催眠作用,复方中酸枣仁油、栀子油起协同作用。

Objective To study the sedative, hypnotic and antiseizure effects of the compound preparation of gardenia oil and jujube seed oil in mice and investigate the interaction of the two drugs in this preparation. Methods The compound preparation was administered intragastrically in mice, whose spontaneous activity was observed along with their tolerance of the preparation after long-term administration. The hypnotic effect of the compound was assessed by investigating the changes in the pentobarbital sodium-induced sleeping. The compound was tested for its antiseizure effect in mice with pentetrazole-induced clonic and tonic convulsion. Diazepam was used as the standard control in all experiments. Results The jujube seed oil, the gardenia oil and their compound all inhibited spontaneous activities of the mice. Compared with diazepam, the compound showed slow action in producing the sedative effect, which increased gradually with prolonged drug administration without obvious drug tolerance responses. The compound and the two oils all showed synergistic action with pentobarbital sodium in inducing sleeping of the mice. Prescription study showed that the compound produced stronger sedative and hypnotic effects than either of the oils. The two oils and the compound did not show significant antiseizure effects in mice. Conclusion The compound of jujube seed oil and gardenia oil has sedative and hypnotic effects in mice, and the two oils in the compound show obvious synergistic effect.