兔VX2肝癌模型制作及肝动脉插管技术改良

Preparation of VX2 rabbit hepatoma models and modification of the hepatic artery catheterization technique

目的应用VX2细胞株制作新西兰兔移植性肝癌模型,并探讨对肝动脉插管技术的改良。方法开腹分别种植VX2瘤块于兔肝左、右叶,制作40只兔VX2肝癌模型。瘤株接种2周后,实验组(26只)采取改良显微外科手术直接行肝动脉插管法行介入治疗,对照组(10只)采用3F微导管经股动脉-肝动脉插管法作对比研究。术前及术后用多层螺旋CT和DSA造影方法进行兔VX2肝癌的影像学观察。结果CT扫描及开腹手术证实瘤株接种成功36只。实验组的插管成功率为88%(23/26);对照组的插管成功率为40%(4/10)。多层螺旋CT平扫兔VX2肝癌表现为低或等密度结节灶;DSA造影示肿瘤呈均一的或结节状染色。结论采用改良显微外科手术直接行肝动脉插管法对兔VX2肝癌行介入治疗成功率较高,实验人员可较大程度地避免辐射,是值得推广的实验方法。

Objective To establish New Zealand rabbit hepatoma models with VX2 cell line, and modify the hepatic artery catheterization technique. Methods Forty New Zealand rabbit models bearing hepatoma were established by implanting VX2 cells into the left and right liver lobes. Two weeks after the tumor cell implantation, 26 rabbits in the experimental group underwent modified hepatic artery catheterization procedures using microsurgical technique, and 10 rabbits in the control group were catheterized with 3F micro-catheter using Seldinger technique. The VX2 hepatomas were observed before and after the catheterization with multi-slice spiral CT scan and digital subtractive angiography （DSA）. Results Tumor growth after the tumor cell implantation was confirmed in 36 rabbits by CT scans and open operations. The success rate of catheterization was 88% （23/26） in the experimental group, and 40% （4/10） in the control group. VX2 hepatomas appeared as hypointense or isointense nodules on multi-slice spiral CT, and hepatic artery angiography showed that VX2 hepatomas had homogeneous or nodular tumor staining. Conclusion The modified hepatic artery catheterization using microsurgical technique has higher success rate than catheterization with 3F micro-catheter by Seldinger technique, and significantly decreases X-ray exposure for the staff undertaking the operations.