兔子宫内膜移植肿瘤模型的建立及其转移性淋巴结的MR观察

Establishment of a rabbit model bearing transplanted endometrial carcinoma and magnetic resonance imaging features of the metastatic lymph nodes

目的建立子宫内膜VX2肿瘤模型并行MR观察。方法采用子宫内膜分离组织包埋法在12只兔子宫内膜制备VX2肿瘤模型,3周后行MR检查,观察肿瘤及其转移性腹膜后淋巴结,并与正常组兔腹膜后淋巴结在大小和信号强度方面进行比较。结果肿瘤原位移植成功率为100%,3周时肿瘤浸润子宫浆膜并发生腹膜后淋巴结转移;MR检查显示肿瘤转移性淋巴结大于正常组淋巴结,但两者在信号强度方面无显著差异。结论此模型移植成功率高,其转移模式与人子宫内膜肿瘤转移方式类似,为研究新型MR淋巴造影对比剂提供了较理想的大动物模型。

Objective To establish a rabbit model bearing endometrial carcinoma and observe the magnetic resonance imaging （MRI） features of the metastatic lymph nodes. Methods VX2 tumor grafts were orthotopically embedded into the endometrium of the rabbits. Three weeks after the implantation, the tumor and the metastatic retroperitoneal lymph nodes were examined with MRI, and the signal intensities and the size of the lymph nodes were compared with those in normal rabbits. Results Orthotopic tumor growth was observed in all the rabbits. Tumor infiltration of the serosa and retroperitoneal lymph node metastasis occurred 3 weeks after tumor implantation. MRI demonstrated obviously lymph node enlargement in the tumor-bearing rabbits as compared with those of normal rabbits, while the signal intensity of the lymph nodes were comparable between them. Conclusion The endometrial carcinoma in this rabbit model well simulate the metastatic behavior of human endometrial carcinoma, and may serve as a good model for testing the efficacy of the contrast agents for MRI of the lymph nodes.