摘要
选用两种有代表性的类胡萝卜素(β-胡萝卜素和黄体素)分别用蛋鸡、山羊的离体小肠细胞进行体外吸收实验。结果表明:β-胡萝卜素和黄体素的吸收均呈浓度依赖性,且β-胡萝卜素吸收率保持稳定(P>0.05);培养温度对蛋鸡离体小肠粘膜细胞类胡萝卜素吸收无显著影响,而极显著地影响了山羊对黄体素和高浓度(≥15μmol/L)β-胡萝卜素的吸收;Tween20对两种类胡萝卜素均有抑制作用,其中对黄体素抑制作用较大;体积分数(φ)为0.3%的Tween20可能阻断了β-胡萝卜素转化为维生素A;0.5mol/L的根皮素抑制了β-胡萝卜素的吸收而对黄体素吸收起促进作用(P<0.01)。
Uptake of βcarotene and lutein by layers and goats was studied in vitro. The result showed uptake of βcarotene and lutein was concentrationdependent and a constant uptake percentage of βcarotene was observed. This suggested carotenoids were uptaken by passive diffusion mechanism. Uptake of carotenoids by cells of layer was temperatureindependent while uptake of lutein and βcarotene at high concentration by goats was affected significantly by incubation temperature. Tween 20 inhibited uptake of carotenoids among which lutein was more heavily inhibited than βcarotene. In addition inhibition of βcarotene and promotion of lutein by phloretin were first reported.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
1997年第4期54-59,共6页
Journal of Nanjing Agricultural University
基金
国家教委回国人员基金