实验犬磨牙根分叉骨缺损应用组织工程骨修复的效果分析

Effects of tissue-engineering bone on repair of bigger furcation bone defects of dogs

目的观察以组织工程方法修复大面积根分叉骨缺损的效果。方法以成骨条件培养液培养实验犬骨髓基质细胞(BMSCs)至第3代并以1×106/cm2的密度与猪的脱矿冻干骨(fdDBM)复合培养后回植于犬磨牙根分叉骨缺损区(2.0cm×1.0cm×0.8cm)。术后3个月与6个月取实验开窗区骨制作组织切片或2mm厚的骨磨片,观察新生骨组织形态及骨钙素与Ⅰ型胶原表达情况并作骨磨片的骨密度分析。所得数据用SAS6.12软件作t检验。结果6个月及3个月实验侧骨缺损完全修复。骨切片显示实验侧开窗区新生骨与正常骨无明显区别且Ⅰ型胶原和骨钙素表达亦同正常。X线骨密度数据显示回植后6个月实验组的骨密度高于对照组及回植3个月者。结论以组织工程骨修复大面积根分叉骨缺损是可行的。

Objective To observe the effects of ossified induced bone marrow stromal cells （BMSCs） and freeze-dried demineralized bone matrix （ fdDBM ） complexes on the repair of the bigger furcation bone defects of dogs. Methods BMSCs from the adult dogs＇ （ n = 6） marrow were cultured in vitro and induced to be osteoblast. The third generation BMSCs were combined with the pig＇s fdDBM,incubated in vitro for 7 days and then implanted into the autologons furcation bone defects （2.0 cm × 1.0 cm ×0. 8 cm ） of dog＇ jaws. At 3 or 6 months＇ postimplantation, the dogs were sacrificed and the jaws were harvested. The specimens were processed for gross inspection, bone density examination, histological and immunohistoehemical observation respectively. Results The man-made defects of the dogs＇ jaws were fdled with new bone fully 3 or 6 months later. The osteocalcin and I -type collagen immunohistoehemistry staining demonstrated that the new bone tissue of every group had positive results.6 months later the density values of new bone in BMSCs-fdDBM group were obviously higher than those of the control group and 3 months＇ group（ P 〈 0. 05）. Conclusion It is practicable to use tissue-enjineering bone to repair the bigger furcation bone defects.