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两种心脏停搏液对缺血心肌细胞凋亡及凋亡相关蛋白表达的影响 被引量：1

Effect of two kinds of cardioplegia on cardiomyocyte apoptosis and related protein expressions in rat hearts
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摘要 目的:观察St.Thomas和河豚毒素(Tetrodotoxin,TTX)两种心脏停搏液对缺血再灌注大鼠心肌细胞凋亡及凋亡相关蛋白表达的影响。方法:Wistar大鼠24只,建立Langendorff-Neely离体心脏灌注模型,分为3组(n=8):对照组、STH-2组、TTX组。用TUNEL法检测心肌细胞凋亡指数(Apoptosis index,AI),免疫组化测定凋亡相关蛋白Bcl-2、Bax、p53的表达,Western blot检测Bcl-2蛋白表达。结果:STH-2组AI(11.20±0.79)%高于TTX组(6.92±1.10)%和对照组(1.34±0.23)%(P<0.01)。TTX组Bcl-2的PEI为(22.63±1.10)%高于STH-2组(13.51±0.67)%和对照组(2.52±0.58)%(P<0.01);STH-2组Bax的PEI为(8.04±0.57)%高于TTX组(6.05±0.46)%和对照组(2.14±0.57)%(P<0.01)。TTX组Bcl-2/Bax为3.78±0.41高于STH-2组1.75±0.15(P<0.01)。TTX组Bcl-2的相对蛋白含量为(86.1±0.98)%高于STH-2组(53.2±1.76)%和对照组(23.7±2.61)%(P<0.01)。p53蛋白在各组均未见明显阳性表达。结论:与STH-2比较,TTX能减少缺血再灌注大鼠心肌细胞凋亡数,其抗凋亡的机制可能与上调Bcl-2表达,下调Bax表达,提高Bcl-2/Bax比值有关。 Objective.To explore the effect of two kinds of cardioplegia on cardiomyocyte apoptosis and the related protein expressions in rat hearts. Methods：24 Wistar rats were randomly divided into 3 groups （n=8）：Control group,STH-2 group and TTX group. Isolated rat Langendorff and Neely working heart models were estabished. The changes in cardiomyocyte apoptosis were detected by TUNEL. The expression of Bcl-2 and Bax protein were detected by immunohistochemical assay in cardiomyocyte. The expression of Bcl-2 protein in cardiomyocyte was detected by Western blot. Results：Apoptosis index（AI） in STH-2 group （ 11.20 ± 0.79 ） % was significantly higher than that in TTX group （6.92 ± 1.10） % and Control group （ 1.34 ± 0.23 ） % （P〈0.01）. Bcl-2 protein expression index（PEI） in TTX group（22.63 ±1.10）% increased as compared with STH-2 group （13.51±0.67）% and Control group （2.52 ±0.58）%（P〈0.01）. Bax PEI in STH-2 group （8.04±0.57）%increased as compared with TTX group （6,05 ±0.46）% and Control group （2.14 ±0.57）%（P〈0.01）. Bcl-2/Bax was 3.78 was 0.41 in TTX group,1.75 ±0.15 in STH-2 group. Bcl-2 protein expression in TTX group （86.1 ±0.98）% increased as compared with STH-2 group （53.2± 1.76）% and Control group （23.7 ±2.61）%（P〈0.01 ）. There was no sign of p53 protein expression in all groups. Conclusion：Compared with STH-2,TTX cardioplegia alleviates the apoptosis of rat cardiomyocytes. The mechanism is probably up-regulating expression of Bcl-2 protein,down-regulating expression of bax protein and increasing Bcl-2/Bax.
作者 綦俊 杨双强 刘胜中
机构地区 重庆医科大学附属第一医院心胸外科
出处 《重庆医科大学学报》 CAS CSCD 2008年第9期1047-1049,1070,共4页 Journal of Chongqing Medical University
基金 重庆市科委课题(CSTC 2005AC0068)
关键词 河豚毒素 心肌缺血再灌注损伤 心肌细胞凋亡 BCL-2蛋白 BAX蛋白 TTX Myocardial ischemia reperfusion injury Cardiomytocyte apoptosis Bcl-2 protein Bax protein
分类号 R654.1 [医药卫生—外科学]
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参考文献9

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共引文献12

同被引文献7

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重庆医科大学学报
2008年 第9期

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