小鼠皮肤切创愈合过程中巨噬细胞炎性蛋白-1α的表达及其与损伤时间的关系

An immunohistochemical study on the time-dependent expression of MIP-1αduring healing of skin incised wound in mice

目的:探讨MIP-1α在小鼠皮肤切创损伤愈合过程中的表达分布变化规律及其与损伤时间的关系。方法:小鼠背部制做全层切创,应用免疫组织化学技术及阳性细胞计数法观察伤后小鼠切创组织中不同时程的生前伤(1h～14d)和死后伤(0.5～6h)皮肤创伤局部MIP-1α的表达,并和无切创的小鼠作为对照。结果:在生前损伤组中,MIP-1α最早在伤后6h出现阳性表达,随后表达逐渐增多,在3d达到高峰,其后呈下降趋势。而MIP-1α在早期死后伤组及对照组仅在表皮,皮脂腺,汗腺上皮细胞呈弱阳性表达。结论:MIP-1α在小鼠皮肤切创愈合过程中呈现一定时序性变化,有望作为法医损伤时间推断的一个有用的参考指标。

Objective：To explore the expression of MIP-1α during healing of skin incised wound and the applicability of time-dependent expressions of MIP-1α to determination of wound age. Methods：An incised wound was made in the dorsal skin of mouse. Immunohistochemical and image-analysis techniques were employed in vital skin wounds lh-14d after incision and postmortem incision 0.5-6h after death, and the non-incised mouse skin was used as control. Results：In the vital skin incisions,expression of MIP-1α began at 6h after incision,which .increased subsequently,and peaked at 3d, The quantity of MIP-1α expression decreased and minimized in the specimens aged 14 days. In the non-wounded groups and postmortem incision groups MIP-1α was only detected as weak expression in the epidermic cells,sweat gland cells and endothelial cells. Conclusion：The time-dependent expression of MIP-1α during healing of skin incised wound may be used as a useful reference marker.