两种草坪草愈伤组织的诱导及其分化研究

Callus induction and plantlet regeneration of two sod grasses, Poa pratensis and Zoysia japonica

对肯塔基兰草的种子、幼穗、茎尖和结缕草的幼穗、茎尖的外植体进行愈伤组织诱导和分化研究。结果表明：在诱导培养基中添加３０μｍｏｌ／Ｌ或１０μｍｏｌ／Ｌｄｉｃａｍｂａ＋０．５ｍｇ／ＬＮＡＡ，与添加２，４－Ｄ、ＮＡＡ、Ｐｉｃｌｏｒａｍ的处理相比，肯塔基兰草种子愈伤组织诱导率和分化率都有提高，绿苗率分别达４１．９％和３８．１％；结缕草的幼穗愈伤组织诱导率达２６．８％，绿苗率达１６％。而肯塔基兰草的幼穗、茎尖和结缕草的茎尖愈伤组织诱导和分化都不理想。

Tissue culture of sod grasses, kentucky bluegrass (Poa pratensis) and turf-grass (Zoysia japonica), was done using seed, immature infloresence and bud as explants. The results indicated that the callus induction medium supplemented with 30 μmol/L or 10 μmol/L dicamba plus 0.5 mg/L NAA was much better than that with 2,4-D, NAA and/or picloram, in callus induction and green plantlet regeneration of seeds from kentucky bluegrass, the highest frequencies of green plantlet regeneration were 41.9% and 38.1%, when calluses were induced from medium with 30 μmol/L dicamba and 10 μmol/L dicamba respectively. The frequency of the immature inflorescence-induced callus was 26.8%, and that of green plantlet regeneration was up to 16%. However the response of immature inflorescence and bud of kentucky bluegrass, and bud of turf-grass to in vitro culture needed to be further improved.