TLC法考察阿霉素脂质体的稳定性

Investigation of the stability of adriamycin liposome by TLC

目的:通过以TLC分离阿霉素脂质体各组分及其降解产物,考察其作为阿霉素脂质体稳定性评价方法的适用性以及进行方法改进。方法:分别用4种TLC展开系统进行2次展开,用4-甲氧基苯甲醛显色。结果:以氯仿-甲醇-水-氨水(65∶35∶2.5∶2.5)作展开剂,分离效果较好。结论:TLC法操作简便、重复性好,可用作脂质体质量控制的一种方法。

Objective：To investigate the usability of TLC as a evaluating method for adriamycin liposome stability by separating its composition and degradation products with TLC and improve this method. Methods：The plates were developed twice with four development systems,visualized by g-methoxybenzaldehyde. Results：It was better to separate EPC,cholesterol and their degradation products by using chloroform-methanol-water-ammonia（65 ： 35 ： 2.5 ： 2_5） as the mobile phase Conclusion.TLC could be used for the quality control of the adriamycin liposome due to its simple operation and high reproducibility.