胰岛素在大鼠脊髓损伤治疗中的实验研究

Empirical study on application of insulin in treating traumatic spinal cord injury in rat models

目的:观察胰岛素对急性脊髓损伤是否有保护作用及其可能的机理。方法:60只SD大鼠,雌雄不限,随机分为A组(损伤对照组)和B组(胰岛素组),每组各30只大鼠,Allen法建立脊髓损伤模型,B组给与1IU/kg的胰岛素,1日3次腹腔灌注,连续7d,A组给与等量同次数的生理盐水腹腔灌注,在伤后8h、第3d、第7d、第14d、第28d观察脊髓凋亡细胞,在第1d、第3d、第7d、第14d、第28d观察尼氏体和神经元数量、GAP-43阳性细胞、BBB评分。结果:B组神经元数量、GAP-43阳性细胞、BBB评分在多个时相点要好于A组(P<0.05或P<0.01),凋亡细胞B组明显少于A组(P<0.05或P<0.01)。结论:胰岛素能通过抑制脊髓神经细胞的凋亡坏死、促进神经轴突的修复和再生来达到保护脊髓神经细胞、促进功能恢复的目的。

Objective：To observe neuroprotective effects of insulin on traumatic spinal cord injury in rat models and discuss its potential mechanisms. Methods：60 SD rats were randomly divided into A group（control group） and B group（insulin group）, with 30 rats in each group,The animal SCI model was established according to method Allen＇s,A group and B group were respectively infused with isotonic Na chloride or 1Iu/kg insulin through abdominal cavity,three times a day,for 7 days. apoptosis neurons were observed on 8 hours,3 days,7 days,14 days,and 28 days after SCI,and tigroid body,neurons,GAP-43 positive cells,and BBB scorn were measured on 1 day,3 days,7 days,14 days,and 28 days after SCI. Results：Neuron number, GAP-43 positive cells,and BBB score in multitude time points were significantly higher in B group than in A group（P〈0.05 or P〈0.01 ）. The number of apoptosis neurons in B group was less than in A group＇s（P〈0.05 or P〈0.01）. Conclusions：Insulin has neuroprotective effects on traumatic spinal cord injury in rat models. It can promote recovery of function by depressing apoptosis of neurons and promoting recovery and regeneration of neuraxon.