一种慢性EAE模型的制备方法

A preparative method of chronic experiment autoimmune encephalomyelitis

[摘要]目的：通过探讨一种慢性实验变态反应性脑脊髓炎（experimental autoimmune encephalomyelitis, EAE）模型的制备方法，为研究多发性硬化的发病机制打下基础。方法：72只近交系清洁级8-10周健康雌性C57BL／6J小鼠。随机分为发病组、佐剂组和空白对照组，各组小鼠又分为发病初期、发病高峰期和慢性期组。发病组用mMOG35-55免疫C57BL／6J小鼠制成慢性EAE动物模型。结果：本实验终止时，发病组C57BL／6J小鼠累计有20／24（83．3％）的小鼠发病，累计有1／24（8．3％）的小鼠死亡，最高临床症状评分达5级，即全身皆瘫后死亡。发病组小鼠第1次免疫后13d内均未发病，14～20d发病，20～24d达高峰，28～32d进入慢性期，部分临床症状可缓解；空白对照组和佐剂组均没有发病。发病小鼠病理学检测有特征性表现。结论：本实验采用人工合成mMOG35-55与自制完全弗氏佐剂混合而成抗原乳剂，成功建立了慢性EAE小鼠模型。本实验所复制的慢性EAE模型具有发病率高、死亡率低、模型稳定的特点，可用于研究多发性硬化。

Objective To explore the model of chronic experimental autoimmune encephalomyelitis （ EAE ） for the further study of multiple sclerosis. Methods A total of 72 female SPF C57BL/6J mice （inbred strain, aged 8 - 10 weeks） , were randomly divided into an EAE group, a blank group and an adjuvant group, and each group was divided into 3 subgroups： an onset group, a peak group and a chronic phase group. The EAE group was immuned with mMOG35-55. Results At the end of the study, and 83.3% of the mice in EAE group suffered the onset, and 8.3% of the mice died. The highest clinical score reached grade 5 , namely paralysis of the whole body and then death. In the EAE group, after being immuned firstly, the mice were all anosis during the first 13 days. They got ill on the third week, and in about 20-24 days the clinical symptom reached the peak, and in 28-32 days the chronic phase arrived, when parts of the clinical symptoms got relieved. On the contrary, both the adjuvant group and the blank group were healthy all the time. Characteristic appearance was detected in the EAE group. Conclusion Antigen emulsion, mixture of artificially synthesized mMOG35-55 and complete Freund＇ s adjuvant can successfully induce chronic EAE in the mice. The model of EAE duplicated in our study has the characteristics of high incidence, low death rate and stability, which can be used to carry out further research on multiple sclerosis.