鬼臼毒素抗胃癌细胞株SGC 7901作用的实验研究

Effect of podophyllotoxin on human gastric cancer cell line SGC 7901

目的:研究鬼臼毒素抗胃癌SGC 7901细胞株的作用,为鬼臼毒素用于临床治疗胃癌提供依据。方法:不同浓度的鬼臼毒素(0,5,10,20和50mg/L)处理SGC7901细胞后,采用MTT比色法和平板克隆形成实验检测细胞的增殖;采用流式细胞术检测细胞周期分布和细胞凋亡;裸鼠成瘤实验检测鬼臼毒素对SGC7 901细胞体内致瘤能力的影响。结果:鬼臼毒素呈时间与剂量依赖性抑制SGC 7901细胞生长,明显降低SGC 7901细胞平板克隆,显著诱导SGC7901细胞凋亡,并使SGC 7901细胞阻滞于G2/M期,还能显著降低SGC 7901细胞裸鼠移植瘤的体积及质量。结论:鬼臼毒素能抑制胃癌SGC 7901细胞的生长,诱导SGC 7901细胞凋亡,并能抑制GC 7901细胞的体内致瘤能力,此为鬼臼毒素临床治疗胃癌提供了科学依据。

Objective To investigate the effect of podophyllotoxin on human gastric cancer cell line SGC 7901 cells in vitro and in vivo, and to provide evidence for treatment of gastric cancer with podophyllotoxin. Methods SGC 7901 cells were treated with podophyllotoxin at various concentrations for different time. MTT assay, colony formation assay, and flow cytometry were performed to determine the effect of podophyllotoxin on the growth and apoptosis of SGC 7901 cells in vitro. Furthermore, xenograft experiment was performed to detect the effect of podophyllotoxin on the in vivo tumorigenicity of SGC 7901 cells. Results Podophyllotoxin inhibited the growth of SGC 7901 cells in a concentration- and time-dependent manner, decreased the colony formation rate of the cells, induced SGC 7901 cell apoptosis, arrested SGC 7901 cells creased the volume and weight of SGC 7901 cell xenografts at G2/M phases of cell cycle, and dein nude mice. Conclusion Podophyllotoxin inhibits the growth of human gastric cancer SGC 7901 cells, induce the apoptosis of SGC 7901 cells in vitro, and inhibit the in vivo tumorigenicity of SGC 7901 cells, which provides evidence for treatment of gastric cancer using podophyllotoxin