期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

拟南芥MAP65-6活体探针的构建 被引量：1

Construction live probe of MAP65-6 from Arabidopsis
下载PDF
导出
摘要 植物细胞中有许多重要的成束的微管结构,如周质微管、细胞质丝束、早前期带、纺锤体微管和成膜体微管等.生化研究已经证实微管相关蛋白MAP65家族的成员具有交联微管使之成束的能力.本文构建了GFP-融合蛋白植物表达载体(pMAP65-6-GFP),并将其转入拟南芥,研究MAP65-6的定位与功能.获得了MAP65-6 C-末端与GFP融合表达的拟南芥转基因株系.运用荧光显微镜初步研究了转基因株系植株和细胞的GFP荧光信号.结果显示,MAP65-6主要呈现细胞质定位,获得的活体探针可以用于MAP65-6和微管功能的进一步研究. Bundled microtubules （MTs） are usually observed in major MT structures in plant cells, cortical microtubules （CMTs）, cytoplasmic strands, preproband （PPB）, spindle and phragmoplast. The MAP65 family has been biochernically identified as an MT cross-bridge factor. Plasmid pMAP65-6-GFP were constructed in this paper and, transferred into in Arabidopsis to detect the functions and locations of MAP65-6. Transgenic lines in which GFP was fused to the C - terminus of the Arabidopsis MAP65 - 6 were obtained and has been analysed with fluorescent protein aided live-cell visualizations. The results showing that MAP65-6 has a cytoplast location and, such live probes can be used for further studies on the functions of MAP65 -6 and MTs.
作者 梁峰 朱智芳 杨清
机构地区 商丘师范学院生命科学系 南京农业大学生命科学学院
出处 《商丘师范学院学报》 CAS 2008年第9期96-99,共4页 Journal of Shangqiu Normal University
关键词 拟南芥 细胞骨架 微管 活体探针 微管相关蛋白 Arabidopsis cytoskeleton microtubules （MTs） live probe microtubule- associated proteins （MAPs）
分类号 Q78 [生物学—分子生物学]
  • 相关文献

参考文献11

  • 1Granger, C L, and Cyr, R J. Microtubule reorganization in tobacco BY - 2 cells stably expressing GFP - MBD [ J ]. Planta, 2000, 210:502 -509.
  • 2Timmers, A C, Niebel, A, Balague, C, et al. Differential localisation of GFP fusions to cytoskeleton - binding proteins in animal, plant, and yeast cells [ J ]. Green- fluorescent protein. Protoplasma,2002, 220: 69- 78.
  • 3Abe, T, and Hashimoto, T. Altered microtubule dynamics by expression of modified alpha - tubulin protein causes right - handed helical growth in transgenic Arabidopsis plants [ J]. Plant J ,2005, 43:191 -204.
  • 4Kordyum, E L, Shevchenko, G V, Yemets, A I, et al. Application of GFP technique for cytoskeleton visualization onboard the International Space Station [ J ]. Acta Astronaut,2005, 56 : 613 - 621.
  • 5Kim, B R, Nam, H Y, Kim, S U, et al. Normalization of reverse transcription quantitative - PCR with housekeeping genes in rice [J] . Biotechnol Lett,2003, 25:1869 - 1872.
  • 6Van Damme, D, Van Poucke, K, Boutant, E, et al. In vivo dynamics and differential microtubule -binding activities of MAP65 proteins [J]. Plant Physiol, 2004,136: 3956-3967.
  • 7Mao, T, Jin, L, Li, H, et al. Two microtubule- associated proteins of the Arabidopsis MAP65 family function differently on microtubules [ J ~. Plant Physiol,2005,138 : 654 - 662.
  • 8Mao, G, Chan, J, Calder, G, et al. Modulated targeting of GFP - AtMAP65 - 1 to central spindle microtubules during division [J]. Plant J,2005,43: 469-478.
  • 9Li, H, Mao, T, Zhang, Z, et al. The AtMAP65 -1 cross- bridge between microtubules is formed by one dimer [J]. Plant Cell Physio1,2007,48:866 - 874.
  • 10Vanstraelen, M, Van Damme, D, De Rycke, R, et al. Cell cycle- dependent targeting of a kinesin at the plasma membrane demarcates the division site in plant cells [ J ]. Curr Biol,2006,16:308 -314.

共引文献2

同被引文献32

  • 1Barroso C, Chan J, Allan V, Doonan J, Hussey P, Lloyd C (2000). Two kinesin-related proteins associated with the cold stable cytoskeleton of carrot ceils: characterization of a novel kinesin, DcKRP120-2. Plant J, 24 (6): 859-868
  • 2Caillaud MC, Dubreuil G, Quentin M, Perfus-Barbeoch L, Lecomte P, de Almeida Engler J, Abad P, Rosso MN, Favery B (2008a). Root-knot nematodes manipulate plant cell functions during a compatible interaction. J Plant Physiol, 165 (1): 104-113
  • 3Caillaud MC, Lecomte P, Jammes F, Quentin M, Pagnotta S, Andrio E, de Almeida Engler J, Marfaing N, Gounon P, Abad P, Favery B (2008b). MAP65-3 microtubule-associated protein is essential for nematode-induced giant cell ontogenesis in Arabidopsis. Plant Cell, 20 (2): 423-437
  • 4Chan J, Jensen CG, Jensen LC, Bush M, Lloyd CW (1999). The 65-kDa carrot microtubule-associated protein forms regularly arranged filamentous cross-bridges between microtubules. Proc Natl Acad Sci USA, 96 (26): 14931-14936
  • 5Chan J, Mao G, Smertenko A, Hussey PJ, Naldrett M, Bottrill A, Lloyd CW (2003). Identification of a MAP65 isoform involved in directional expansion of plant cells. FEBS Lett, 534 (1-3): 161-163
  • 6Chan J, Rutten T, Lloyd C (1996). Isolation of microtubuleassociated proteins from carrot cytoskeletons: a 120 kDa map decorates all four microtubule arrays and the nucleus. Plant J, 10 (2): 251-259
  • 7Chang HY, Smertenko AP, Igarashi H, Dixon DP, Hussey PJ (2005). Dynamic interaction of NtMAP65-1a with microtubules in vivo. J Cell Sci, 118 (Pt 14): 3195-3201
  • 8Criqui MC, Parmentier Y, Derevier A, Shen WH, Dong A, Genschik P (2000). Cell cycle-dependent proteolysis and ectopic overexpression of cyclin B1 in tobacco BY2 cells. Plant J, 24 (6): 763-773
  • 9Gaillard J, Neumann E, Van Damme D, Stoppin-Mellet V, Ebel C,Barbier E, Geelen D, Vantard M (2008). Two microtubuleassociated proteins of Arabidopsis MAP65s promote antiparallel microtubule bundling. Mol Biol Cell, 19 (10): 4534-4544
  • 10Genschik P, Criqui MC, Parmentier Y, Derevier A, Fleck J (1998). Cell cycle-dependent proteolysis in plants: identification of the destruction box pathway and metaphase arrest produced by the proteasome inhibitor MG132. Plant Cell, 10 (12): 2063-2076

引证文献1

二级引证文献1

商丘师范学院学报
2008年 第9期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部