HPLC法测定妇炎消片中大黄素及大黄酚的含量

Determination the Content of Emodin and Chrysophanol in Fuyanxiao Tablets by HPLC

目的:建立妇炎消片中大黄素与大黄酚的HPIC测定方法。方法:色谱柱为岛津C_(18)(250 mm×4.6 mm,5μm),流动相为甲醇-0.1%磷酸(83∶17);流速为1 ml·min^(-1),检测波长为254 nm。结果:大黄素在进样量0.19～0.95μg范围内呈良好线性关系,r=0.999 7。平均回收率为98.35%,RSD为1.53%;大黄酚进样量在0.09～0.44μg范围内呈良好线性关系,r= 0.999 8,平均回收率为97.67%,RSD为1.20%。结论:本方法简便、准确、灵敏。可用于妇炎消片的质量控制。

Objective： To establish an HPLC method for determination the content of emodin and chrysophanol in Fuyanxiao tablets. Method： The SHIMADZU VP-ODS C18 （250 mm × 4.6 mm,5 μm） was used with the methanol-0.1% phosphoric acid solution （83： 17） as mobile phase, the flow rate was 1.0 ml·min-1 ,and the detection wavelongth was at 254 nm. Result： The linear range of emodin was 0.19 -0.95μg（ r ： 0.999 7）, the average recovery was 98.35% （RSD 1.53% ） ;the linear range of chrysophanol was 0.09 -0. 44μg（ r=0.999 8）the average recovery was 97.67% （RSD 1.20% ） . Conclusion： the method is simple and reliable, it can be used for quality control of Fuyanxiao tablets.