乙肝病毒表面抗原基因对番茄的遗传转化及转基因番茄植株的获得

Transformation of HBsAg Gene into Tomato and Production of Transgenic Tomato Plants

利用农杆菌介导法将乙肝病毒表面抗原基因转入番茄,获得了转基因植株.经GUS组织化学染色、PCR扩增以及PCR-Southern检测,证明乙肝病毒表面抗原基因已转入番茄基因组中.这为番茄乙肝口服疫苗的研究奠定基础.

The HBsAg gene was transformed into tomato via Agrobacterium tumefaciens and some transgenic tomato plants were obtained. GUS staining, PCR analysis and PCR-Southern analysis demonstrated that the HBsAg gene had been integrated into the tomato genome. These results might lay down a foundation for the study of oral vaccine of HBV by tomato transformed by HBsAg gene.