摘要
利用农杆菌介导法将乙肝病毒表面抗原基因转入番茄,获得了转基因植株.经GUS组织化学染色、PCR扩增以及PCR-Southern检测,证明乙肝病毒表面抗原基因已转入番茄基因组中.这为番茄乙肝口服疫苗的研究奠定基础.
The HBsAg gene was transformed into tomato via Agrobacterium tumefaciens and some transgenic tomato plants were obtained. GUS staining, PCR analysis and PCR-Southern analysis demonstrated that the HBsAg gene had been integrated into the tomato genome. These results might lay down a foundation for the study of oral vaccine of HBV by tomato transformed by HBsAg gene.
出处
《西南大学学报(自然科学版)》
CAS
CSCD
北大核心
2008年第7期78-83,共6页
Journal of Southwest University(Natural Science Edition)
基金
福建省科技厅重点科技资助项目(2004N026)
福建省自然基金资助项目(B0410009)
福建省教育厅科技资助项目(JB03125)
关键词
乙肝表面抗原基因
转化
转基因番茄
hepatitis B surface antigen gene (HBsAg gene)
transformation
transgenic tomato