反相高效液相色谱法测定小鼠血浆中德氮吡格

Determination of TNBG in Plasma by RP-HPLC

采用固相萃取反相高效液相色谱法测定小鼠血浆中的德氮吡格。血浆样品经过C18固相萃取小柱净化后，在Lichrospher C18色谱柱（4．6mm×250mm，5μm）上，以甲醇-0．1％三乙胺水溶液（pH6．5，体积比90：10）为流动相，流速1．0mL／min，检测波长260nm对德氯吡格进行测定。实验结果表明，德氮吡格的血药浓度在1～20μg／mL范围内与峰面积呈良好线性关系（r≥0．995），平均回收率为101．36％，高中低三浓度的日内精密度均小于4％，日间精密度均小于8％，最低检测限为0．4μg／mL，该方法简便、快速、准确度高、可靠性好。

A specific and simple high-performance liquid chromatographic （HPLC） method was developed for the determination of TNBG in plasma with UV-visible detection. HPLC separation on Lichrospher C18 （4.6 mm×250 mm,5μm） was carried out with an isocratic mobile phase of 90% methanol in 100 mL methanol-deionized water （pH 6.5）,containing 0.1% triethyl amine and detection at 260 nm. The retention time of TNBG was less than 6 min. The linearity was good over the concentration range 1-20 μg/mL （r≥0.995）. The recoveries were in the range 98.1-104.5% （n=5）,and the inter- and intra-day RSD were less than 8.0%. The detection limit was 0.4 μg/mL. This novel and rapid method can be employed in a routine clinical setting.