产前束缚应激对大鼠子代的影响

Effect of prenatal restraint stress on the offspring of rats

目的:观察产前束缚应激对子代大鼠海马组织脑源性神经生长因子(BDNF)的表达及学习行为的影响。方法:将孕鼠随机分为应激组和对照组,应激组孕鼠在妊娠的第13～19天给予束缚应激。观察两组孕鼠所产雄性仔鼠1月龄时的学习记忆和行为活动,并用免疫组织化学方法检测仔鼠海马组织切片中BDNF的表达。结果:应激组雄性仔鼠海马BDNF的表达低于相应对照组(t=3.377,P=0.004),学习记忆成绩低于对照组(t=3.838,P=0.002),水平运动得分高于对照组(t=2.372,P=0.028),垂直运动得分高于对照组(t=2.625,P=0.016)。结论:产前应激可能减少仔鼠海马组织中BDNF的表达,并影响仔鼠的学习记忆和行为活动。

Objective： To detect the effect of prenatal restraint stress on learning, memory, action and on the expression of brain - derived neurotrophic fact in hippocampus of the off springs. Methods： The pregnant rats were randomly divided into stress group and control group. Stress group was inflicted each day of pregnancy from day 13 to day 19. Then one - month - old male rats were examined to analyze the learning, memory and action. Then the rats were examined by immunohistochemical method to analyze the expression of BDNF in hippocampus. Results： The male stress group had lower expression of BDNF than the control group （t = 3.377, P = 0. 004） ; The learning and memory scores of the stress group were lower than the control group （t = 3. 838, P = 0. 002） ; The gallery level activity scores were higher than the control group （t = 2.372, P = 0. 028） ; The vertical activity scores were higher than the control group （ t = 2.625, P = 0. 016）. Conclusion ： Prenatal stress may decrease the expression of BDNF in hippocampus and affect learning, memory and action of the offspring.