正交试验在怀山药组织培养中的应用

Application of orthogonal design in tissue culture of Dioscorea opposite

目的:研究植物生长物质种类和浓度、蔗糖含量对怀山药茎段形成不定芽和诱导小块茎的影响。方法:选用怀山药的茎段为材料,采用正交设计研究蔗糖、6-苄基腺嘌呤(6-BA)、萘乙酸(NAA)对丛生芽和小块茎诱导的影响,对所得实验数据进行极差分析、方差分析。结果与结论:茎段诱导丛生芽最适宜的培养基为MS+6-BA 1mg.L-1+NAA 1 mg.L-1+蔗糖3%,各因素影响程度依次为蔗糖>6-BA>NAA;诱导小块茎最适宜培养基为MS+6-BA 1.5 mg.L-1+NAA 1.5 mg.L-1+蔗糖5%,各因素影响程度依次为蔗糖>6-BA>NAA。

Objective： To study the effects of different factors on buds and microtuber. These factors included plant growth substances and sucrose. Method： stems were selected as explants. The effects of three kinds of factors were studied by orthogonal design method including sucrose, 6-BA, NAA on the buds and microtuber induction. The data were analyzed with range analysis and vafiance analysis. Result and conclusion： The optimal media to induce many buds from stems wereMS ＋ 6-BA 1 mg· L^-1 ＋ NAA 1 mg· L^-1 ＋ sucrose 3%, the effect of the three factors was in sequence of sucrose 〉 6-BA 〉 NAA . The optimal media to induce micro- tuber from stems were MS ＋ 6-BA 1.5 mg · L^-1 ＋ NAA 1.5 mg· L^-1 ＋ sucrose 5%, the effect of the three factors was in sequence of sucrose 〉6-BA 〉 NAA.