二妙丸类方抗湿热证痛风有效部位群指纹图谱比较研究

Fingerprint comparison of effective parts of Ermiao wan categorized formula in anti-gouty syndrome of damp and heat

目的:为探索类方方剂配伍物质基础变化特征,研究二妙丸类方(二妙丸、三妙丸、四妙丸及加味四妙丸)配伍物质基础的关联。方法:建立加味四妙丸抗湿热证痛风有效部位群HPLC指纹图谱,利用对照品确定指纹图谱共有峰成分、组方药有效部位色谱峰归属以及有效部位群共有峰的组方药有效部位来源,测定二妙丸类方抗湿热证痛风有效部位群指纹图谱,比较指纹图谱共有峰成分特征及配伍变化。结果:加味四妙丸有效部位群有60个共有峰,分别来源于组方药生物碱、黄酮、有机酸部位。基础方与类方间有效部位群指纹图谱共有峰在数目及相对峰面积比值方面呈现一定的变化规律。结论:二妙丸及其类方有共同的物质基础,从基础方到各类方,抗湿热证痛风物质基础的配伍变化具有相关性。

Objective： To study the correlation in the material foundation in compatibility of Ermiao wan categorized formulas （Ermiaowan, Sanmiaowan, Simiaowan and modified Simiaowan） and to explore its characteristics changes. Method： Determined the fingerprint of the effective parts of Ermiao wan categorized formulas in anti-gouty syndrome of damp and heat and compared the composi- tion features and compatibility changes of total peaks by establishing the HPLC fingerprint and using reference substances to identify the ingredients of the common peaks, the ascription and the source of the chromatographic peaks. Result： The effective parts of modified Simiao Wan had 60 total peaks which come from the sites of alkaloids, flavonoids, organic acids. There were some change principles in the number of total peaks and relative peak area ratio between basic formula and categorized formulas. Conclusion： Ermiaowan categorized formulas have same substance- foundation and there are some correlations in the compatibility changes.