夜香牛全草的生物活性成分研究

Studies on bioactive constituents of whole herbs of Vernonia cinerea

目的:研究广西产夜香牛生物活性化学成分。方法:用鼠性肾上腺髓质嗜铬细胞瘤PC-12细胞活性跟踪筛选,柱色谱方法分离化合物,谱学方法鉴定化合物结构。结果:分离得到4个化合物,根据谱学数据分析鉴定其结构分别为木脂素类化合物(+)-lirioresinol树脂醇B(1),甾醇类化合物豆甾醇(2)和豆甾醇3-O-β-D-葡萄糖苷(3),以及4-sulfo-benzocyclobutene(4);用神经细胞体外模型试验,检测了分离得到化合物的NGF诱导活性。结论:化合物1,3,4为首次从该属植物中分离得到,化合物4为新的天然产物。

Objective： To study the constituents of the whole herbs of Vernonia cinerea, by bio-activity guided isolation with PC-12 model. Method： The constituents were separated by column chromatography and the structures were elucidated by spectroscopic methods. Result： Four compounds were identified to be （ ＋ ）-lirioresinol B （1）, stigmasterol （2）, stigmasterol-3-O-β-D-glucoside （3）, 4-sulfo-benzocyclobutene （4）, and their NGF inducing activity were also investigated. Conclusion： Compounds 1, 3, 4 were isolated from this genus for the first time, and compound 4 was identified as a new natural product. Compounds 1, 3, 4 showed cyto- toxicity on PC-12, and compounds 2, 3, 4 showed inhibition activity. Compound 4 showed a specific effect on the survival of TrkA fibroblasts, and resulted in the inducing NGF activity.