印度人参根提取物对免疫低下小鼠免疫功能的影响

Immunomodulatory effect of Indian ginseng root extract on immunodeficiency mice

目的:印度人参根提取物(IRRE)对免疫低下小鼠的免疫调节作用。方法:给免疫低下小鼠灌胃印度人参根提取物5,2.5,0.85 g.kg-1体重,观察其对免疫低下小鼠免疫功能的影响。结果:印度人参根提取物可减缓由环磷酰胺引起的生长缓慢和脏器指数下降,对小鼠迟发型变态反应和抗体生成细胞有增强作用,在刀豆球蛋白A(ConA)刺激的淋巴细胞转化实验中,印度人参根提取物各剂量组显著高于环磷酰胺组(P<0.05)。各剂量组骨髓DNA含量、血清一氧化氮(NO)含量显著高于空白组和环磷酰胺组,但磷酸酶活性变化不大。结论:印度人参根提取物对由环磷酰胺导致的巨噬细胞吞噬功能下降和脾脏损伤有一定恢复能力,能有效保护环磷酰胺所导致的骨髓抑制作用。

Objctive： To investigate the immunoregulation action of Indian ginseng root extract（IRRE） in immunodeficiency mice. Method： Immunodeficiency mice were fed IRRE with doses of 5, 2.5, 0.85 g · kg^-1 for 15 days before their immune function were observed. Result： IRRE could relieve retarded growth and viscera index drop of mice induced by CP, could heighten the response of delayed hypersensitivity and the amount of antibody in serum. The proliferation of ConA-induced mitogenic response for spleen lymphocyte were also increased, compared with the CP group （P 〈 0.05 ）. IRRE could enhance the content of marrow DNA and NO of serum with no change of acid phosphatase and alkalinity phosphatase. Conclusion： IRRE could enhance the phagocytosis of celiac macrophage, protect the spleen from damage and improve marrow inhibition in immunodeficiency mice.