葛根素对2型糖尿病大鼠脂肪组织ADRP基因表达的影响

Effects of puerarin on ADRP gene expression in fatty tissue of type 2 diabetes mellitus rats

目的:观察葛根素(Pue)对实验性2型糖尿病(2-DM)大鼠脂肪组织中脂肪分化相关蛋白(ADRP)基因mRNA表达的影响。方法:Wistar大鼠喂以高糖高脂饮食伴尾静脉注射小剂量链脲佐菌素(STZ)25 mg.kg-1复制2-DM大鼠模型,将造模大鼠随机分为模型组(腹腔注射丙二醇)和3个Pue治疗组(腹腔注射40,80,160 mg.kg-1),另选10只大鼠为正常组。各组大鼠连续腹腔注射6周后,空腹取材,测定空腹血糖(FBG)、血清胰岛素(FINS)水平,并计算胰岛素抵抗指数(IR);同时以RT-PCR法检测脂肪组织ADRP基因mRNA的表达。结果:与2-DM模型组比较,Pue高、中剂量组大鼠脂肪组织ADRP基因mRNA表达显著降低(P<0.05);Pue高、中、低剂量组大鼠FBG,IR及Pue高、中剂量组大鼠FINS显著降低(P<0.05或P<0.01)。结论:Pue可通过降低2-DM大鼠ADRP基因mRNA表达,降低胰岛素抵抗水平,从而降低2-DM大鼠血糖。

Objective： To observe the effects of puerarin on ADRP gene mRNA expression in fatty tissue of type 2 diabetes mellitus rats （T2DM）. Method： Wiastar rats of T2DM model were made by feeding with high glucose and fat diet and injecting with small dose of streptozocin （25 mg ·kg^-1 ）. 40 model rats were randomly divided into model control group and three puerarin groups （40, 80, 160 mg · kg^-1）, another 10 rats were selected as normal control group. FBG and FINS were measured to calculate IR after rats were injected consecutively for 6 weeks. The level of ADRP gene mRNA in fatty tissue was determined by RT-PCR after rats were injected eight weeks. Result： Compared with model control group, high and middle dosage of puerarin can decreased ADRP gene mR- NA expression in fatty tissue obviously, FBG, IR level in each puerarin group and FINS in high and middle dosage puerarin groups decreased obviously. Conclusion： Puerarin can decrease the blood glucose level of T2DM by downregulating ADRP mRNA expression and depressing the insulin resistance.