枇杷LFY同源基因植物表达载体构建及其功能分析

Construction of expression vector and function analysis of ejLFY,a LEAFY-like gene of loquat

为研究枇杷LFY同源基因的功能,构建了含枇杷LFY同源基因ejLFY的植物表达载体pBI121-ejLFY,并转入到农杆菌GV3101中。利用花序侵染法将该基因导入拟南芥,经抗性筛选、PCR检测表明获得了25个转化株系。与对照相比,大部分转基因植株明显早花,成花时间可提早1周左右,莲座叶数目减少2-3片;部分植株侧生花序全部转变成单独的花,从莲座叶中抽出的花枝也全部转变为单独的花;少量转化株系花器官异常,不能形成种子。这表明枇杷LFY同源基因在其成花过程中起重要作用,为了解枇杷的成花分子机制奠定了基础。

In order to analyse the function of ejLFY, a LEAFY-like gene of loquat, the plant expression vector was constructed by inserting into pBI121 plasmid vector, then transformed into Arabidopsis thaliana by Agrobacterium tumefaciens strain GV3101 using the floral dip method and 25 transformed lines were obtained with the selection of Kanamicin and the detection of PCR. Most of the transgenic A. thaliana plants ectopically expressing ejLFY showed early-flowering pbenotypes,which correlated with a significant decrease in rosette leaf production. Some of the transformed plants produced flowers instead of shoots at either axillal7 or accessol7 bud meristems and all the normal intlorescence were directly adjacent to a node converted to flowers. Some of the transgenie plantlets can not produce the seeds for their abnormal flower organs. These results primarily proved the important function of ejLFY in the flowering process.