马铃薯花药培养再生植株的诱导与鉴定

Dihaploid induction and evaluation of potato

文章采用讷16、克新13、克新17、克新18、花525和波C共6个马铃薯品种为试验材料,研究了不同的基因型、不同浓度的植物生长调节剂和蔗糖对马铃薯花药培养的影响,并对马铃薯花药再生植株进行了细胞学、形态学和农艺性状的观察与鉴定。结果表明,基因型中波C和讷16具有较强的再生能力;MS+NAA0.5 mg.L-1+2,4-D0.5mg·L-1+KT0.5 mg·L-1适合愈伤组织的形成;分化培养基为MS+NAA0.2 mg·L-1+6-BA2.0 mg.L-1+ZT1.0 mg·L-1;蔗糖浓度为6%时愈伤组织的诱导率最高;移栽的双单倍体植株在生长势、叶色、薯型等性状与对照相比存在差异。

Six genotypes of potatoes, Ne16, Kexin13, Kexin17, Kexin18, Hua525 and BoC, were used to study the factors affecting the potato anther culture, including hormone and sucrose. The agronomy, cytology and morphology of dihaploids of potato were examined. The results showed that the regeneration abilities of the genotypes Nel6 and BoC were better than that of others. MS＋NAA 0.5 mg·L^-1＋2, 4-D 0.5mg·L^-1＋KT 0.5 mg·L^-1 was comparatively more suitable for callus induction. The better callus differentiation medium was MS＋NAA 0.2 mg·L^-1＋ 6-BA 2.0 mg·L^-1＋ZT 1.0 mg·L^-1. The culture medium with 6% sucrose gave the highest callus induction percentage. The differences of the dihaploids and their parental tetraploid cultivars were noted in the traits such as plant vigor, leaf color and tuber shape.