HPLC测定不同产地川党参中党参炔苷的含量

Determination of lobetyolin in root of Codonopsis tangshen from various cultivation areas by high-performance liquid chromatography

目的：分析不同产地的川党参中党参炔苷的含量，为川党参的质量标准建立提供参考。方法：采用高效液相色谱分析，Supelco Discovery C18色谱柱（4．6mm×250mm，5μm）；乙腈-0.5％醋酸水（20：80）为流动相，检测波长268nm。结果：24批不同种与不同产地的党参中党参炔苷含量介于0.0403～0.9667mg·g^-1。结论：该方法操作简便，结果稳定可靠，可用于川党参的质量评价。

Objective： To determine lobetyolin in the root of Codonopsis tangshen from the various cultivation areas. Method： A Supelco Discovery C18 Column （4. 6 mm ×250 mm, 5 μm） was used with acetonitrile-0. 5% acetic acid in water （20： 80） as the mobile phase and UV detection was at 268 nm. Result： Twenty-four batches of the samples were analyzed. The content of lobetyolin ranged from 0. 040 3-0. 966 7 mg · g^-1. Conclusion： The method was simple, reproducible and reliable. It can be used to control the quality of C. tangshen.