两种福尔马林试剂对大鼠炎性痛诱导的行为学比较

Comparison of Nocifensive Behaviors Evoked by Imported and Domestic-made Formalin in Rats

建立福尔马林疼痛模型,研究不同产地或不同生产批次福尔马林试剂对大鼠炎性痛诱导的效果,并以Dubuisson评分方法进行记录比较.结果表明,国产组第二时相疼痛时间明显短于进口组,二者之间存在极显著差异(p<0.01).不同生产批次的福尔马林试剂对大鼠的炎性痛诱导效果无显著差异.建议在经济条件允许的情况下使用进口封装的福尔马林制作疼痛模型,同一产家的福尔马林试剂在12个月内可以安全使用.

The present study was designed to investigate the potential difference of effects evoked by imported and domestic-made formalin. Nocifensive behaviors （lifting and licking） were evaluated by Dubuisson scoring using in-house software following subcutaneous injection of formalin into the hindpaw. The results showed that group received indigenous formalin displayed significantly less time of lifting/licking in the second phase of the formalin test than group with imported formalin. However, indigenous formalin that was made in different time evoked very similar behavioral response. It suggests that either imported or domestic-made formalin should be consistently, but not alternatively, used. If domestic-made formalin is used, it would be better used within 12 months after formalin solution was made.