CD4^＋CD25^＋T细胞的扩增方法与临床应用

The proliferation and clinical application of CD4^＋ CD25^＋ regulatory T-cell

CD4^＋CD25^＋T细胞是最重要的一类调节性T细胞（Tr）。体内固有CD4^＋CD25^＋T细胞的自然扩增率极低，不能满足临床治疗的需要。通过采用FoxP3基因转染技术、阻断细胞活化信号、DC诱导、加入细胞因子等方法，对CD4^＋CD25^＋T细胞的数量和功能进行扩增，使其在器官移植、自身免疫性疾病和肿瘤免疫等领域具有广泛的临床应用前景。

CD4^＋ CD25^＋ T cells are the most important cells among the regulatory T-cells. It is known that the natural proliferation of natural-occurring CD4^＋ CD25^＋ Tcell is very low, and is not satisfied for the clinical therapies. However, the quantity and function of CD4^＋CD25^＋ T could be increased through FoxP3 gene transfection, actiration signal blockade, dendritil cell induction, and cytokine stimulation. Thus the clinical application of CD4^＋CD25^＋ T in organ trartsplantation, autoimmune disease and tumor immunity areas will be possitble.