黄连、黄柏配方颗粒及其饮片汤剂的盐酸小檗碱含量比较

Comparison of Content of Berberine Hydrochloride in Dispensing Granules and Slice Decoctions of Rhizoma Coptidis and Cortex Phellodendri

目的通过测定黄连、黄柏配方颗粒及其饮片煎煮制得的汤剂的盐酸小檗碱含量,评价不同厂家黄连、黄柏配方颗粒的质量。方法随机抽取不同厂家生产的数批黄连、黄柏配方颗粒,采用柱层析分离干扰成分,紫外分光光度法测定含量。结果A厂黄连、黄柏配方颗粒相当于原生药含盐酸小檗碱含量分别为4.83%～5.17%和0.31%～0.92%;B厂黄连、黄柏配方颗粒相当于原生药含盐酸小檗碱含量分别为1.92%～3.49%和0.46%～0.51%。黄连、黄柏含量测定方法平均回收率分别为100.93%和101.70%。结论不同厂家产品及其饮片汤剂三者盐酸小檗碱含量比较差异非常显著(P<0.01)。

Objective To compare the content of berberine hydrochloride in Rhizoma Coptidis and cortex Phellodendri dispensing granules produced by different pharmaceutical factories and their slice decoctions. Methods To stochastically take some of cortex Phellodendri and Rhizoma Coptidis dispensing granules produced by different pharmaceutical factories. The products were assayed by column chromatography combined with UV- spectrophotometry. Results The cortex Phellodendri and Rhizoma Coptidis dispensing granules produced by A factory was equal to the original crude drugs containing berberine hydrochloride from 4.83% -5. 17% ,0.31% -0.92% and it by B factory was from 1.92%-3.49% ,0.46% -0.51%, respectively. The average recovery rates of cortex Phellodendri and Rhizoma Coptidis content determination method were 100.84% and 101.3%. Conclusion The content of berberine hydrochloride is obviously different in dispensing granules produced by different pharmaceutical factories and slice decoctions（P 〈 0.01）.