摘要
目的用高效液相色谱(HPLC)法测定复方柳安咖注射液中水杨酸钠、安替比林和咖啡因含量。方法采用Kromasil C18柱(250mm×4.6mm,5μm),以乙腈-0.1%磷酸二氢钾溶液(15∶85)为流动相,流速为1.0mL/min,检测波长为272nm。结果水杨酸钠、安替比林和咖啡因进样量分别在0.7000~2.100μg(r=0.9999),0.2000~0.6000μg(r=0.9999)和0.1000~0.3000μg(r=0.9999)范围内与峰面积线性关系良好,平均回收率分别为99.1%,99.4%,99.7%,RSD分别为0.6%,0.5%,0.4%(n=9)。结论HPLC法可用于同时测定复方柳安咖注射液中水杨酸钠、安替比林和咖啡因的含量。
Objective To establish the HPLC method for the determination of three components in compound sodium salicylate, antipyrine and caffeine injection, Methods The Kromasil C,s column(250 mm×4.6 mm, 5μm) was used with acetonitrile-0.1% potassium dihydrogen phosphate solution (15:85) as the mobile phase. The flow rate was 1.0 mL/min. The detection wavelength was at 272 nm. Results The linear ranges of sodium salieylate, antipyrine and caffeine were 0. 700 0 -2. 100μg( r=0. 999 9), 0. 200 0 -0.600 0μg( r =0. 999 9)and 0, 1000-0.3000μg(r=0.9999),The average recovery rates were 99. 1%,99.4% and 99.7%,with RSD 0.6%,0.5%,0.4%(n=9). Conclusion The method can be used for the determination of three components in compound sodium salicylate, antipyrine and caffeine injection.
出处
《中国药业》
CAS
2008年第18期39-40,共2页
China Pharmaceuticals