银马解毒颗粒水提取工艺研究

Study on Water Extracting Technology for Yinmajiedu Granules

目的优选银马解毒颗粒的水提工艺。方法采用L9(34)正交试验设计,以加水量、煎煮时间、煎煮次数、醇沉浓度为主要考察因素,以金银花的绿原酸和甘草的甘草酸铵总含量作为选择指标。结果水提醇沉制备工艺影响因素影响大小依次为煎煮次数、煎煮时间、醇沉浓度、加水量;最佳提取工艺为处方药材加10倍量水,每次煎煮1h,煎煮2次,醇沉浓度为40%。结论按优选的工艺条件放大试验,工艺稳定,结果满意。

Objeetive To optimize the water extracting technology for Yinmajiedu Granules. Methods L9（3^4） of orthogonal experiments was used. The water amounts, boiling hours, boiling times and precipitating concentration of alcohol were taken as main factors. The total contents of chlorogenic acid in Flosaslonicerae and ammonium glycyrrhizinate in Glycyrrhiza uralensis Fisch. were as valuable markers. Results The influential effects of factors were as follows： C 〉 B 〉 D 〉 A（A： added water volumn, B： decoction time, C： decoction frequency, D： precipitation concentration of alcohol）. The optimizing preparation conditions were A3B2C3D1, i. e. added 10 times water, decoction for 3 times, 1 h each time and added alcohol to 40%. Conclusion The technology is stable and the results are satisfying when magnifying the experiment according to the optimized conditions.