槲皮素对人肝癌细胞耐药株SMMC7721/VCR逆转作用的实验研究

Study on Reversion Effect of Quercetin on Drug-resistance of Human Liver Cancer Cell SMMC7721/VCR

目的探讨槲皮素（Que）对人肝癌细胞耐长春新碱（VCR）耐药株（SMMC7721／VCR）逆转作用的影响。方法应用四甲基偶氮唑盐（MTT）法分析Que对人肝癌细胞敏感株（SMMC7721／S）、耐药株（SMMC7721／VCR）的抑制作用；Que与VCR联合作用时，SMMC7721／S对VCR的增敏作用及对SMMC7721／VCR耐药性的逆转作用。结果SMMC7721／S、SMMC7721／VCR对VCR的IC50值分别为（1．16±0．06）mg／L和（13．28±0．35）mg／L。SMMC7721／VCR对VCR耐药倍数为11．45倍。Que对两细胞株的生长有明显的抑制作用。25．0～50．0μmol／L终浓度的Que在体外能够明显提高SMMC7721／VCR对VCR的敏感性。结论Que能够部分逆转SMMC7721／VCR细胞的耐药性。它可作为有效的多药耐药逆转剂，与其它化疗药物联合应用于肿瘤的化学治疗。

Objective To explore the effect of Vincristine （VCR） of human liver cancer cell line （SMMC7721/VCR） reversed by Quercetin. Methods MTT assay was adapted for examining the inhibitory effects of Queretin on liver cancer sensitive line （SMMC7721/S） and to VCR resistance line（SMMC7721/VCR）. We also analyzed that the chemo-sensitization of SMMC7721/VCR when Quercetin was cmbined with VCR. Results The IC50 values of SMMC7721/S and SMMC7721/VCR to VCR were （1.16±0.06）mg/L and （13.28±0. 35）mg/L, respectively. It was 11.45 folds that resistance of SMMC7721/VCR to VCR. Quercetin could significantly inhibit the growth of SMMC7721/S and SMMC7721/VCR cells. Comparing with SMMC7721/S,25. 0- 50.0 μmol/L Quercetin in vitro could obviously enhance the sensitivity of SMMC7721/VCR to VCR. Conclusion Quercetin could partly revise the resistance of SMMC7721/VCR. It may become a reversing agent of effective multi-drug resistance and may be applied to chemotherapy of cancer when Quercetin combined with chemodrugs.