摘要
目的 :测定和比较日本血吸虫浙江株和安徽株 NADH脱氢酶 1( NADH1)和细胞色素 C氧化酶 1( Cytc1)基因部分序列。方法 :用 PCR方法从特定的引物扩增出 NADH1和 Cytc1DNA基因 ,然后进行测序。结果 :获得两株日本血吸虫成虫 NADH1和 Cytc1基因及其序列。结论 :两株日本血吸虫成虫 NADH1基因及其部分序列除第 382位核苷酸不同 ( C/T)外 ,其余的均相同。两株日本血吸虫成虫 Cytc1基因及其部分序列完全相同。
AIM:To determine and compare NADH dehydrogenase1( NADH1) and cy- tochrome C oxidase1( Cytc1) partial gene sequences of Zhejiang and Anhui isolates of Schistosoma japonicum.METHODS:NADH1and Cytc1genes were amplified by PCR using special primers corresponding with 4 4 1and 370 bp of the genomic DNAs,and subsequently sequenced. RE- SUL TS:NADH1and Cytc1gene sequences were obtained from the two isolates. CONCLUSION: The partial sequences of the NADH1genes were essentially identical for both isolates with only one nucleotide difference ( C/ T) at position 382 . The Cytc1sequences were virtually identical for the two isolates.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
1997年第6期378-381,共4页
Chinese Journal of Parasitology and Parasitic Diseases
关键词
日本血吸虫
NADH
脱氢酶
细胞色素C
氧化酶
序列
Schistosom a japonicum,N ADH dehydrogenase1,cytochrom e C oxidase1,PCR,sequence.