日本血吸虫 NADH脱氢酶 1和细胞色素 C氧化酶 1基因部分序列(英文)

PARTIAL SEQUENCE OF NADH DEHYDROGENASE1AND CYTOCHROME C OXIDASE1GENE OF SCHISTOSOMA JAPONICUM

目的 :测定和比较日本血吸虫浙江株和安徽株 NADH脱氢酶 1( NADH1)和细胞色素 C氧化酶 1( Cytc1)基因部分序列。方法 :用 PCR方法从特定的引物扩增出 NADH1和 Cytc1DNA基因 ,然后进行测序。结果 :获得两株日本血吸虫成虫 NADH1和 Cytc1基因及其序列。结论 :两株日本血吸虫成虫 NADH1基因及其部分序列除第 382位核苷酸不同 ( C/T)外 ,其余的均相同。两株日本血吸虫成虫 Cytc1基因及其部分序列完全相同。

AIM:To determine and compare NADH dehydrogenase1( NADH1) and cy- tochrome C oxidase1( Cytc1) partial gene sequences of Zhejiang and Anhui isolates of Schistosoma japonicum.METHODS:NADH1and Cytc1genes were amplified by PCR using special primers corresponding with 4 4 1and 370 bp of the genomic DNAs,and subsequently sequenced. RE- SUL TS:NADH1and Cytc1gene sequences were obtained from the two isolates. CONCLUSION: The partial sequences of the NADH1genes were essentially identical for both isolates with only one nucleotide difference ( C/ T) at position 382 . The Cytc1sequences were virtually identical for the two isolates.