囊尾蚴病患者红细胞免疫粘附功能的研究

STUDY ON THE FUNCTION OF RED CELL IMMUNE ADHERENCE SYSTEM IN CYSTICERCOSISPATIENTS

目的 :探讨囊尾蚴病患者红细胞免疫粘附功能 ( RCIA)变化及其机制。方法 :用酵母菌标记技术检测囊尾蚴病患者红细胞表面 C3b受体活性及 RCIA调节因子活性 ;用琼脂单扩散法测血清补体 C3含量 ,用聚乙二醇 -紫外分光法测血清 CIC含量。结果 :囊尾蚴病患者红细胞膜 C3b受体花环率及血清 RCIA增强因子活性明显低于正常对照组 ,而红细胞膜 IC花环率 ,血清中免疫粘附抑制因子活性及血清中 CIC含量较正常对照组明显升高。结论 :囊尾蚴病患者红细胞膜 C3b受体活性受损 ,清除 CIC能力下降。

AIM:To investigate the changes and mechanism of red cell immune adherence function (RCIA) in cysticercosis patients.METHODS:The activity of C3b receptor on red blood cell membrance and the activity of regulated factor of RCIA were determined by yeast labelled technique.The levels of complement C3and circulated immune complex in sera from cysticer- cosis patients were determined by gel diffusion and PEG- UV spectrophotometry,respective- ly.RESUL TS:The rate of red blood cell C3b receptor rosette and the activity of enhancing factor of RCIA in the cysticercosis patientgroup were markedly lower than those of the con- trol group,while the rate of immune complex rosette,the activity of inhibiting factor of RCI- Aand the level of CIC in the patientgroup were significantly higher than those of the control group.CONCLUSION:In cysticercosis patients,the activity of C3b receptor in red cell mem- brance was affected and the ability of eliminating CIC was reduced.