甘肃省以医院为基础的子宫颈癌及宫颈高度内瘤变患者HPV感染及型别分布

Human Papillom Avirus Type Distribution Analysis of Invasive Carcinoma of Cervix Uteri and High Degree Cervical Intraepithelial Neoplasia on the Hospital Analysis of Gansu Province

目的研究以医院为基础的浸润性子宫颈癌(ICC)和宫颈高度内瘤变(≥CINII)组织病理标本中人乳头瘤病毒(HPV)的感染及型别分布情况、HPV的多型别感染与宫颈病变的关系,预测HPV疫苗在本地区的潜在影响。方法兰州市户籍并常住,2005—2006年间在本院住院诊治,术前未进行任何化学或放射治疗做出诊断的42例ICC或≥CINII的活组织标本。在中国医学科学院肿瘤研究所(CICAMS)采用"三明治"技术,由组织病理学专家对HE染色后的切片进行双盲独立阅片,运用PCR技术对病变部位进行HPV-DNA检测。结果在42例患者中,CINⅡ11例,CINⅢ11例,ICC20例;感染HPV41例,感染率为97.62%;单一型别感染率为90.24%,多型别感染率为9.76%。HPV型别分布:HPV-16、58、31、33、52、18、51、59、68、6,其中,HPV-16感染31例,占75.61%,HPV-58感染4例,占9.76%,二者合计为85.37%。在20例ICC患者中,鳞癌17例,其中16例为HPV-16型别单一感染。

OBJECTIVES This research is based on the hospital＇ s tissue pathology sample of high degree cervical intraepithelial neoplasia（ICC）, and high degree cervical intraepithelial neoplasia（≥CINⅡ）,through the analysis of human papillom avirus （HPV） infection and the HPV type distribution, and the relationship between HPV multi-type infection and the uterine cervix pathological changes within these samples, to forecast the potential impact of HPV bacterin within the research areas. METHOD Take the 42 cases diagnosed ICC or ≥CINⅡ living tissue samples, which were come from the patients of Lanzhou City registered citizens, who have been in hospital and be treated in Gansu Provincial Maternity and Child Care Hospital within year of 2005 to 2006, and did not take any chemical or radiology treatment before medical operation. Within The Cancer Institute of Chinese Academy of Medical Sciences, the ＂Sandwich＂ technology be used, and the experts of tissue pathology have read the HE dyed slice under the situation Without any information. The pathology change part have been taken the HPV-DNA examination by PCR technology. RESULTS Within the 42 cases, 11 cases are CIN Ⅱ, CINm are 11 cases, ICC are 20 cases, HPV infection 41 cases, is the 97.62% of total samples. Single type infection occupied 90.24%, and the multi-type infection is 9.76%. HPV distribution： HPV-16, 58, 31, 33, 52, 18, 51, 59, 68, 6, among of them, HPV-16 infection are 31 cases, is 75.61% of total, and HPV-58 infection are 4 eases, is 9.76% of total. The subtotal of HPV-16 and HPV-58 is occupied 85.37% of total samples. Within the 20 cases of ICC, there are 17 eases squamous carcinoma. Among these 17 eases, there are 16 cases are the HPV-16 single type infection.