维A酸对人肺癌细胞系A549中尿激酶及其受体表达的影响

THE EFFECTS OF ALLTRANS RETINOIC ACID ON THE EXPRESSION OF UROKINASE AND ITS RECEPTOR GENE IN HUMAN LUNG CANCER CELLS A549

探讨了全反式维甲酸（ＲＡ）对人肺癌Ａ５４９细胞中尿激酶（ｕＰＡ）及其受体（ｕＰＡＲ）基因调控作用。在细胞培养过程中用２．５μｍｏｌ／Ｌ和１０μｍｏｌ／ＬＲＡ处理Ａ５４９细胞，两种浓度的ＲＡ均可抑制Ａ５４９细胞生长，以１０μｍｏｌ／ＬＲＡ作用明显；ＤＮＡ－ＲＮＡ斑点杂交检测显示，ＲＡ对Ａ５４９细胞ｕＰＡ及ｕＰＡＲ基因表达具有剂量依赖性调控作用，１０μｍｏｌ／ＬＲＡ可显著抑制Ａ５４９细胞中ｕＰＡ及ｕＰＡＲｍＲＮＡ表达，而２．５μｍｏｌ／ＬＲＡ则无明显效应。结果证明，一定浓度的ＲＡ可调节肿瘤细胞中ｕＰＡ和ｕＰＡＲｍＲＮＡ表达，ＲＡ对肿瘤细胞ｕＰＡ和ｕＰＡＲ表达的下调作用有助于进一步阐明ＲＡ抑制肿瘤细胞浸润和转移的机制。

The effects of alltrans retinoic acid (RA) on the modulation of urokinase plasminogen activator (uPA) and its receptor (uPAR) mRNA were studied in human lung cancer A549 cell line. DNARNA dot blot hybridization assays indicated that the expression of uPA and uPAR mRNA had dosedependent regulating action by RA, and were inhibited obviously by 10 μmol/L RA. This finding suggested that uPA mRNA was regulated by RA at a certain concentration. The inhibitory effects of RA on uPA and uPAR gene expressions in this study may help to clarify the mechanism of RAinduced inhibition effect on tumor invasion and metastasis.