医用灭菌剂量超声波对人骨肉瘤组织的灭活作用

EXPERIMENTAL RESEARCH ON THE LETHAL EFFECT OF BACTERICIDAL DOSE OF ULTRASONIC WAVES ON HUMAN OSTEOSARCOMA TISSUE IN VITRO

目的研究医用灭菌剂量超声波在不同介质和作用时间内，对人骨肉瘤组织的杀伤和灭活作用。方法采用瘤组织碱性磷酸酶染色分级计数和瘤细胞存活度计数方法，观测１０００Ｗ，１８０ｋＨｚ超声波在生理盐水（实验组Ⅰ）或９５％酒精溶液（实验组Ⅱ）中０～３５ｍｉｎ内对新鲜人骨肉瘤组织标本的杀伤和灭活作用，并与未用超声波的生理盐水（阴性对照组）和９５％酒精（参比对照组）进行比较。结果超声作用２０ｍｉｎ时，置于生理盐水中的瘤组织标本，其酶染色较淡，范围较小，平均瘤细胞存活数明显减少（与阴性对照组比较，Ｐ＜００１）；同时置于９５％酒精溶液中的瘤组织标本，基本无酶染色可见，且几乎无存活瘤细胞（与阴性和参比对照组比较，Ｐ＜０００１～００１）。瘤组织标本在不同溶液介质中超声作用和密闭静置２０ｍｉｎ时，其平均瘤细胞存活数依次为：阴性对照组＞实验组（Ⅰ）＞参比对照组＞实验组（Ⅱ）（组间比较，Ｐ＜０００１～００５）。结论医用灭菌剂量超声波在生理盐水中一定时限内对人骨肉瘤组织具有直接杀伤和灭活作用，并可促进９５％酒精溶液对瘤组织的灭活作用。

PURPOSE To study the lethal effect of bactericidal dose of ultrasonic waves through different mediums and within the effective time frame on human osteosarcoma tissue.METHODS The lethal effect of 1000W and 18 kHz ultrasonic waves on human osteosarcoma tissue in normal saline (group Ⅰ) or 95% alcohol(group Ⅱ) within 0～35 minutes was studied by the method of graded counting of tumor tissue by AKP staining and cell count of surviving tumor cells. Comparisons were made between normal saline not treated by ultrasonic waves (negative controls) and 95% alcohol (parameter controls).RESULTS After 20 minutes of ultrasonic application, the tumor tissue specimen immersed in normal saline had lighter enzyme staining ,smaller area, and marked decrease of mean number of surviving tumor cells(compared to negative controls, P<0.01. The specimens in 95% alcohol did not show any staining, and no surviving cells (compared to negative and parameter controls the P<0.001～0.01).CONCLUSIONS Within a definite time frame, a bactericidal dose of ultrasonic wave application can be lethal to osteosarcoma tissue in normal saline, and can potentiate the lethal effect of 95% alcohol on osteosarcoma tissue.